################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BpmI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HindIII HpaI KpnI MamI MboI MfeI MflI MluI MroI MseI MslI MunI NaeI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 402 GGCGCC G[GA]^CG[CT]C Alw44I 844 GTGCAC G^TGCAC ApaLI 844 GTGCAC G^TGCAC AscI 338 GGCGCGCC GG^CGCGCC BalI 791 TGGCCA TGG^CCA BbeI 402 GGCGCC GGCGC^C BbsI 559 GTCTTC GAAGAC(2/6) BcgI 276 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 229 CTAG C^TAG BglI 733 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 15 GCTCAGC GC^T[ACGT]AGC Bpu1102I 15 GCTCAGC GC^T[ACGT]AGC BpuAI 559 GTCTTC GAAGAC(2/6) BsaHI 402 GGCGCC G[GA]^CG[CT]C BsiWI 455 CGTACG C^GTACG BsmFI 770 GGGAC GGGAC(10/14) BsrFI 335 ACCGGC [GA]^CCGG[CT] BssHII 339 GCGCGC G^CGCGC CelII 15 GCTCAGC GC^T[ACGT]AGC Cfr10I 335 ACCGGC [GA]^CCGG[CT] EarI 750 CTCTTC CTCTTC(1/4) HaeII 402 GGCGCC [GA]GCGC^[CT] HgaI 58 GACGC GACGC(5/10) Hin1I 402 GGCGCC G[GA]^CG[CT]C HincII 529 GTCAAC GT[CT]^[GA]AC HindII 529 GTCAAC GT[CT]^[GA]AC HinfI 118 GACTC G^A[ACGT]TC KasI 402 GGCGCC G^GCGCC Ksp632I 750 CTCTTC CTCTTC(1/4) MaeI 229 CTAG C^TAG MaeIII 779 GTAAC ^GT[ACGT]AC MluNI 791 TGGCCA TGG^CCA MscI 791 TGGCCA TGG^CCA NarI 402 GGCGCC GG^CGCC NcoI 590 CCATGG C^CATGG PaeR7I 783 CTCGAG C^TCGAG PflMI 628 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 118 GACTC GAGTC(4/5) PpuMI 770 GGGACCC [GA]G^G[AT]CC[CT] PstI 643 CTGCAG CTGCA^G SbfI 642 CCTGCAGG CCTGCA^GG SfoI 402 GGCGCC GGC^GCC SmlI 783 CTCGAG C^T[CT][GA]AG Sse8387I 642 CCTGCAGG CCTGCA^GG StuI 128 AGGCCT AGG^CCT TaqI 784 TCGA T^CGA TthHB8I 784 TCGA T^CGA Van91I 628 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 783 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### ApaI 638 GGGCCC GGGCC^C 660 GGGCCC AvaII 293 GGACC G^G[AT]CC 771 GGACC BseRI 277 GAGGAG GAGGAG(10/8) 433 GAGGAG BsiEI 680 CGGCCG CG[GA][CT]^CG 722 CGGCCG BsrBI 406 CCGCTC CCGCTC(-3/-3) 490 CCGCTC BsrI 464 ACTGG ACTGG(1/-1) 882 CCAGT EagI 680 CGGCCG C^GGCCG 722 CGGCCG EclXI 680 CGGCCG C^GGCCG 722 CGGCCG Eco52I 680 CGGCCG C^GGCCG 722 CGGCCG EcoNI 239 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 300 CCTCACCGAGG EcoT14I 473 CCAAGG C^C[AT][AT]GG 590 CCATGG HphI 206 TCACC GGTGA(8/7) 302 TCACC KspI 605 CCGCGG CCGC^GG 737 CCGCGG MaeII 533 ACGT A^CGT 761 ACGT MboII 560 TCTTC GAAGA(8/7) 751 TCTTC NlaIII 264 CATG CATG^ 591 CATG SacII 605 CCGCGG CCGC^GG 737 CCGCGG SfcI 220 CTACAG C^T[GA][CT]AG 643 CTGCAG SmaI 615 CCCGGG CCC^GGG 667 CCCGGG StyI 473 CCAAGG C^C[AT][AT]GG 590 CCATGG XmaCI 615 CCCGGG C^CCGGG 667 CCCGGG XmaI 615 CCCGGG C^CCGGG 667 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI BanI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV RsaI Sau96I ScrFI TseI