################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeI MfeI MluI MroI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### BalI 410 TGGCCA TGG^CCA BamHI 403 GGATCC G^GATCC BanI 648 GGTGCC G^G[CT][GA]CC BanII 601 GGGCTC G[GA]GC[CT]^C BbsI 502 GAAGAC GAAGAC(2/6) BbvCI 314 CCTCAGC CCTCAGC(-5/-2) BmyI 601 GGGCTC G[AGT]GC[ACT]^C BpmI 881 CTCCAG CTGGAG(16/14) BpuAI 502 GAAGAC GAAGAC(2/6) BsaAI 849 TACGTG [CT]AC^GT[GA] BsaBI 700 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 248 GAGACC GGTCTC(1/5) BseRI 604 CTCCTC GAGGAG(10/8) Bsp1286I 601 GGGCTC G[AGT]GC[ACT]^C BstAPI 672 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 55 CCAATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 865 TTTAAA TTT^AAA EaeI 410 TGGCCA [CT]^GGCC[GA] EcoNI 606 CCTCCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 108 ATGCAT ATGCA^T HaeIII 411 GGCC GG^CC HphI 486 GGTGA GGTGA(8/7) MaeII 850 ACGT A^CGT MamI 700 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 410 TGGCCA TGG^CCA MscI 410 TGGCCA TGG^CCA NsiI 108 ATGCAT ATGCA^T TaqI 774 TCGA T^CGA TthHB8I 774 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 639 GTAC GT^AC 845 GTAC AlwI 404 GATCC GGATC(4/5) 403 GGATC BglII 348 AGATCT A^GATCT 712 AGATCT BsaJI 252 CCCTGG C^C[ACGT][ACGT]GG 407 CCCTGG BsiYI 593 CCGACTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 606 CCTCCAACAGG BslI 593 CCGACTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 606 CCTCCAACAGG BspHI 149 TCATGA T^CATGA 530 TCATGA BsrI 736 CCAGT ACTGG(1/-1) 883 CCAGT EarI 665 CTCTTC CTCTTC(1/4) 337 GAAGAG HincII 377 GTCAAC GT[CT]^[GA]AC 745 GTTGAC HindII 377 GTCAAC GT[CT]^[GA]AC 745 GTTGAC Ksp632I 665 CTCTTC CTCTTC(1/4) 337 GAAGAG PleI 589 GACTC GAGTC(4/5) 595 GACTC PstI 497 CTGCAG CTGCA^G 670 CTGCAG RcaI 149 TCATGA T^CATGA 530 TCATGA RsaI 639 GTAC GT^AC 845 GTAC SspI 562 AATATT AAT^ATT 819 AATATT TspRI 507 CAGTG CA[GC]TG[ACGT][ACGT]^ 884 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI ApoI BbvI BsmAI BssKI BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI HinfI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I XhoII