Restriction map for
pF1KB7625
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 36 CGCG CG^CG
AflII 195 CTTAAG C^TTAAG
AgeI 473 ACCGGT A^CCGGT
Asp700I 617 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 846 GGTCC G^G[AT]CC
BanI 852 GGCACC G^G[CT][GA]CC
BbsI 697 GAAGAC GAAGAC(2/6)
BfaI 280 CTAG C^TAG
BfrI 195 CTTAAG C^TTAAG
BmyI 851 GGGCAC G[AGT]GC[ACT]^C
BpuAI 697 GAAGAC GAAGAC(2/6)
BsgI 22 GTGCAG GTGCAG(16/14)
BsiEI 44 CGGTCG CG[GA][CT]^CG
BsmI 240 GAATGC GAATGC(1/-1)
Bsp1286I 851 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 806 TGTACA T^GTACA
BspMI 461 ACCTGC ACCTGC(4/8)
BsrFI 473 ACCGGT [GA]^CCGG[CT]
BsrGI 806 TGTACA T^GTACA
BssSI 584 CACGAG CACGAG(-5/-1)
BstUI 36 CGCG CG^CG
Cac8I 147 GCGGGC GC[ACGT]^[ACGT]GC
Cfr10I 473 ACCGGT [GA]^CCGG[CT]
DdeI 236 CTCAG C^T[ACGT]AG
DraI 553 TTTAAA TTT^AAA
DraIII 854 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 331 GATATC GAT^ATC
FauI 147 GCGGG CCCGC(4/6)
HaeIII 211 GGCC GG^CC
HgaI 34 GACGC GACGC(5/10)
HindIII 400 AAGCTT A^AGCTT
MaeI 280 CTAG C^TAG
MslI 889 CACGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 145 CAGCGG C[AC]G^C[GT]G
MvnI 36 CGCG CG^CG
NcoI 692 CCATGG C^CATGG
PinAI 473 ACCGGT A^CCGGT
PstI 463 CTGCAG CTGCA^G
SfaNI 301 GATGC GCATC(5/9)
SmlI 195 CTTAAG C^T[CT][GA]AG
SpeI 279 ACTAGT A^CTAGT
SspBI 806 TGTACA T^GTACA
XcmI 81 CCAGTTCCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 617 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 498 GTAC GT^AC
807 GTAC
AluI 401 AGCT AG^CT
411 AGCT
BamHI 39 GGATCC G^GATCC
259 GGATCC
BcnI 849 CCGGG CC^[GC]GG
870 CCCGG
BsaWI 42 TCCGGT [AT]^CCGG[AT]
473 ACCGGT
BsrI 484 ACTGG ACTGG(1/-1)
81 CCAGT
BstNI 494 CCTGG CC^[AT]GG
507 CCAGG
BstXI 836 CCACGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
888 CCACGAAGGTGG
Cfr13I 210 GGGCC G^G[ACGT]CC
846 GGTCC
DsaI 692 CCATGG C^C[GA][CT]GG
836 CCACGG
EcoRII 494 CCTGG ^CC[AT]GG
507 CCAGG
EcoT14I 569 CCAAGG C^C[AT][AT]GG
692 CCATGG
MboII 381 GAAGA GAAGA(8/7)
697 GAAGA
MvaI 494 CCTGG CC^[AT]GG
507 CCAGG
NciI 849 CCGGG CC^[GC]GG
870 CCCGG
PleI 232 GACTC GAGTC(4/5)
863 GACTC
RsaI 498 GTAC GT^AC
807 GTAC
Sau96I 210 GGGCC G^G[ACGT]CC
846 GGTCC
StyI 569 CCAAGG C^C[AT][AT]GG
692 CCATGG
TaqI 47 TCGA T^CGA
941 TCGA
TthHB8I 47 TCGA T^CGA
941 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AlwI ApoI BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstYI DpnII Fnu4HI FokI HapII
HinfI HpaII HphI ItaI MaeIII
MboI MflI MnlI MseI MspI
MwoI NdeII NlaIII NlaIV Sau3AI
ScrFI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII