Restriction map for
pF1KB7632
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AcsI AcyI AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BglI
BglII BlnI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MfeI MflI MluI
MluNI MscI MslI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoII
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 405 GTCTAC GT^[AC][GT]AC
AccII 332 CGCG CG^CG
AccIII 121 TCCGGA T^CCGGA
AflII 285 CTTAAG C^TTAAG
AhdI 882 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 712 GATCC GGATC(4/5)
AspEI 882 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 21 GGCACC G^G[CT][GA]CC
BbsI 30 GAAGAC GAAGAC(2/6)
BbvCI 1007 CCTCAGC CCTCAGC(-5/-2)
BbvI 779 GCTGC GCAGC(8/12)
BfrI 285 CTTAAG C^TTAAG
BlpI 412 GCTGAGC GC^T[ACGT]AGC
BpmI 559 CTGGAG CTGGAG(16/14)
Bpu1102I 412 GCTGAGC GC^T[ACGT]AGC
BpuAI 30 GAAGAC GAAGAC(2/6)
BsaAI 785 CACGTA [CT]AC^GT[GA]
BsaBI 130 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 121 TCCGGA [AT]^CCGG[AT]
BseAI 121 TCCGGA T^CCGGA
BsmAI 562 GAGAC GTCTC(1/5)
BspEI 121 TCCGGA T^CCGGA
BspMI 597 GCAGGT ACCTGC(4/8)
BsrFI 44 ACCGGC [GA]^CCGG[CT]
BstUI 332 CGCG CG^CG
CelII 412 GCTGAGC GC^T[ACGT]AGC
Cfr10I 44 ACCGGC [GA]^CCGG[CT]
DsaI 987 CCATGG C^C[GA][CT]GG
EaeI 493 CGGCCA [CT]^GGCC[GA]
Eam1105I 882 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 1026 CCCGC CCCGC(4/6)
HaeII 242 AGCGCC [GA]GCGC^[CT]
HgaI 399 GCGTC GACGC(5/10)
MamI 130 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 121 TCCGGA T^CCGGA
MvnI 332 CGCG CG^CG
NcoI 987 CCATGG C^CATGG
NdeI 566 CATATG CA^TATG
PflMI 658 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 905 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 410 CAGCTG CAG^CTG
SfaNI 811 GATGC GCATC(5/9)
SfcI 407 CTACAG C^T[GA][CT]AG
SmaI 622 CCCGGG CCC^GGG
TfiI 118 GAATC G^A[AT]TC
TseI 779 GCTGC G^C[AT]GC
Van91I 658 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 90 CCAGACGACTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 622 CCCGGG C^CCGGG
XmaI 622 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AclI 429 AACGTT AA^CGTT
691 AACGTT
BseRI 740 CTCCTC GAGGAG(10/8)
70 GAGGAG
BsrBI 490 GAGCGG CCGCTC(-3/-3)
571 GAGCGG
BstF5I 206 CATCC GGATG(2/0)
198 GGATG
Cac8I 775 GCTCGC GC[ACGT]^[ACGT]GC
1025 GCCCGC
FokI 206 CATCC GGATG(9/13)
198 GGATG
HphI 254 TCACC GGTGA(8/7)
635 TCACC
MspA1I 35 CCGCTG C[AC]G^C[GT]G
410 CAGCTG
MwoI 48 GCTTTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
471 GCGAAAGGGGC
NlaIII 745 CATG CATG^
988 CATG
PaeR7I 577 CTCGAG C^TCGAG
938 CTCGAG
PleI 172 GACTC GAGTC(4/5)
738 GACTC
Psp1406I 429 AACGTT AA^CGTT
691 AACGTT
SapI 226 GAAGAGC GCTCTTC(1/4)
538 GAAGAGC
StuI 260 AGGCCT AGG^CCT
289 AGGCCT
Tsp509I 547 AATT ^AATT
1080 AATT
XhoI 577 CTCGAG C^TCGAG
938 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BanII BcnI BmyI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI NciI NdeII NlaIV RsaI
Sau3AI Sau96I ScrFI SmlI StyI
TaqI Tru9I Tsp45I TspRI TthHB8I