Restriction map for
pF1KB7663
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstPI BstUI
BstYI CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MflI
MluI MroI MspA1I MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NspI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 335 AAATTT [GA]^AATT[CT]
Alw44I 725 GTGCAC G^TGCAC
AocI 82 CCTCAGG CC^T[ACGT]AGG
ApaLI 725 GTGCAC G^TGCAC
ApoI 335 AAATTT [GA]^AATT[CT]
AseI 144 ATTAAT AT^TAAT
AsnI 144 ATTAAT AT^TAAT
Asp700I 808 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 587 GGACC G^G[AT]CC
BalI 897 TGGCCA TGG^CCA
BclI 768 TGATCA T^GATCA
BsaJI 971 CCATGG C^C[ACGT][ACGT]GG
BsmAI 3 GTCTC GTCTC(1/5)
BsrDI 637 GCAATG GCAATG(2/0)
BssKI 386 CCAGG ^CC[ACGT]GG
Bst1107I 870 GTATAC GTA^TAC
BstBI 815 TTCGAA TT^CGAA
BstNI 386 CCAGG CC^[AT]GG
BstZ17I 870 GTATAC GTA^TAC
Bsu36I 82 CCTCAGG CC^T[ACGT]AGG
DraII 570 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 971 CCATGG C^C[GA][CT]GG
EaeI 897 TGGCCA [CT]^GGCC[GA]
Eco81I 82 CCTCAGG CC^T[ACGT]AGG
EcoO109I 570 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 386 CCAGG ^CC[AT]GG
EcoT14I 971 CCATGG C^C[AT][AT]GG
EcoT22I 542 ATGCAT ATGCA^T
FbaI 768 TGATCA T^GATCA
MfeI 644 CAATTG C^AATTG
MluNI 897 TGGCCA TGG^CCA
MscI 897 TGGCCA TGG^CCA
MslI 702 CACACTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 644 CAATTG C^AATTG
MvaI 386 CCAGG CC^[AT]GG
MwoI 634 GCAGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 971 CCATGG C^CATGG
NsiI 542 ATGCAT ATGCA^T
NspV 815 TTCGAA TT^CGAA
PshBI 144 ATTAAT AT^TAAT
ScrFI 386 CCAGG CC^[ACGT]GG
SfuI 815 TTCGAA TT^CGAA
SpeI 279 ACTAGT A^CTAGT
StuI 691 AGGCCT AGG^CCT
StyI 971 CCATGG C^C[AT][AT]GG
XmnI 808 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 131 GTCTAC GT^[AC][GT]AC
870 GTATAC
AlwI 649 GATCC GGATC(4/5)
389 GGATC
AspHI 725 GTGCAC G[AT]GC[AT]^C
1000 GAGCAC
BbvI 468 GCAGC GCAGC(8/12)
634 GCAGC
BsgI 13 GTGCAG GTGCAG(16/14)
215 GTGCAG
BsiHKAI 725 GTGCAC G[AT]GC[AT]^C
1000 GAGCAC
BstXI 701 CCACACTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCAAGTATCTGG
Cac8I 411 GCAAGC GC[ACGT]^[ACGT]GC
568 GCAGGC
Cfr13I 571 GGCCC G^G[ACGT]CC
587 GGACC
Fnu4HI 468 GCAGC GC^[ACGT]GC
634 GCAGC
HinfI 604 GATTC G^A[ACGT]TC
829 GAATC
ItaI 468 GCAGC GC^[ACGT]GC
634 GCAGC
PstI 466 CTGCAG CTGCA^G
534 CTGCAG
Sau96I 571 GGCCC G^G[ACGT]CC
587 GGACC
SfcI 466 CTGCAG C^T[GA][CT]AG
534 CTGCAG
TaqI 126 TCGA T^CGA
816 TCGA
TfiI 604 GATTC G^A[AT]TC
829 GAATC
TseI 468 GCAGC G^C[AT]GC
634 GCAGC
Tsp45I 422 GTCAC ^GT[GC]AC
521 GTGAC
TthHB8I 126 TCGA T^CGA
816 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BfaI BmyI Bsp1286I BsrI
BstF5I DdeI DpnII FokI HaeIII
HphI MaeI MaeIII MboI MboII
MnlI MseI NdeII NlaIII Sau3AI
SfaNI Tru9I Tsp509I TspRI