Restriction map for
pF1KB7710
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbrPI BciVI BfaI BfrI BglI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MseI
MslI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 395 GTCGAC GT^[AC][GT]AC
AhdI 934 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 532 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 757 CCTGAGG CC^T[ACGT]AGG
AspEI 934 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 563 TGGCCA TGG^CCA
BbeI 1040 GGCGCC GGCGC^C
BbvCI 570 GCTGAGG CCTCAGC(-5/-2)
BcgI 282 CGAGAACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1166 TGATCA T^GATCA
BglII 266 AGATCT A^GATCT
BlpI 1221 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1221 GCTGAGC GC^T[ACGT]AGC
BsaI 624 GGTCTC GGTCTC(1/5)
BsgI 1086 CTGCAC GTGCAG(16/14)
BsmBI 385 CGTCTC CGTCTC(1/5)
BsrBI 172 CCGCTC CCGCTC(-3/-3)
BsrFI 107 GCCGGC [GA]^CCGG[CT]
BsrI 399 ACTGG ACTGG(1/-1)
Bsu36I 757 CCTGAGG CC^T[ACGT]AGG
CelII 1221 GCTGAGC GC^T[ACGT]AGC
Cfr10I 107 GCCGGC [GA]^CCGG[CT]
DraIII 340 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 934 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 966 GAAGAG CTCTTC(1/4)
Eco81I 757 CCTGAGG CC^T[ACGT]AGG
EcoNI 354 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 206 CCATGG C^C[AT][AT]GG
FbaI 1166 TGATCA T^GATCA
HgaI 1154 GCGTC GACGC(5/10)
HindIII 460 AAGCTT A^AGCTT
KasI 1040 GGCGCC G^GCGCC
Ksp632I 966 GAAGAG CTCTTC(1/4)
MluNI 563 TGGCCA TGG^CCA
MscI 563 TGGCCA TGG^CCA
NaeI 107 GCCGGC GCC^GGC
NarI 1040 GGCGCC GG^CGCC
NcoI 206 CCATGG C^CATGG
NgoMIV 107 GCCGGC G^CCGGC
PpuMI 321 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1066 CAGCTG CAG^CTG
SalI 395 GTCGAC G^TCGAC
SapI 966 GAAGAGC GCTCTTC(1/4)
SfoI 1040 GGCGCC GGC^GCC
StyI 206 CCATGG C^C[AT][AT]GG
TfiI 874 GATTC G^A[AT]TC
XcmI 908 CCAGCCGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 1040 GGCGCC G[GA]^CG[CT]C
1153 GGCGTC
AfaI 47 GTAC GT^AC
951 GTAC
ApaI 140 GGGCCC GGGCC^C
225 GGGCCC
AspHI 253 GAGCAC G[AT]GC[AT]^C
411 GAGCAC
AspI 775 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1150 GACGGCGTC
BanI 1040 GGCGCC G^G[CT][GA]CC
1207 GGCACC
BsaHI 1040 GGCGCC G[GA]^CG[CT]C
1153 GGCGTC
BsiHKAI 253 GAGCAC G[AT]GC[AT]^C
411 GAGCAC
BstEII 78 GGTGACC G^GT[ACGT]ACC
1275 GGTGACC
BstPI 78 GGTGACC G^GT[ACGT]ACC
1275 GGTGACC
DraII 139 GGGGCCC [GA]G^G[ACGT]CC[CT]
321 GGGTCCC
DsaI 206 CCATGG C^C[GA][CT]GG
344 CCGTGG
EaeI 563 TGGCCA [CT]^GGCC[GA]
1100 TGGCCG
EcoO109I 139 GGGGCCC [GA]G^G[ACGT]CC[CT]
321 GGGTCCC
EcoO65I 78 GGTGACC G^GT[ACGT]ACC
1275 GGTGACC
HaeII 1040 GGCGCC [GA]GCGC^[CT]
1141 AGCGCC
Hin1I 1040 GGCGCC G[GA]^CG[CT]C
1153 GGCGTC
NlaIII 31 CATG CATG^
207 CATG
PflFI 775 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1150 GACGGCGTC
RsaI 47 GTAC GT^AC
951 GTAC
SmaI 1023 CCCGGG CCC^GGG
1203 CCCGGG
Tth111I 775 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1150 GACGGCGTC
XmaCI 1023 CCCGGG C^CCGGG
1203 CCCGGG
XmaI 1023 CCCGGG C^CCGGG
1203 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I XhoII