################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MaeIII MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AfaI 600 GTAC GT^AC AflIII 1020 ACACGT A^C[GA][CT]GT Aor51HI 697 AGCGCT AGC^GCT BbeI 207 GGCGCC GGCGC^C BcgI 1059 CGACTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 993 GCTGAGC GC^T[ACGT]AGC Bpu1102I 993 GCTGAGC GC^T[ACGT]AGC BsaI 1120 GAGACC GGTCTC(1/5) BsgI 791 GTGCAG GTGCAG(16/14) BspMI 1229 ACCTGC ACCTGC(4/8) BssHII 296 GCGCGC G^CGCGC BstXI 836 CCAAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 483 AGATCC [GA]^GATC[CT] CelII 993 GCTGAGC GC^T[ACGT]AGC DraIII 187 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 697 AGCGCT AGC^GCT EcoNI 1194 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG KasI 207 GGCGCC G^GCGCC KspI 856 CCGCGG CCGC^GG MboII 941 TCTTC GAAGA(8/7) MflI 483 AGATCC [GA]^GATC[CT] NarI 207 GGCGCC GG^CGCC PvuII 745 CAGCTG CAG^CTG RsaI 600 GTAC GT^AC SacII 856 CCGCGG CCGC^GG SbfI 1230 CCTGCAGG CCTGCA^GG SexAI 188 ACCAGGT A^CC[AT]GGT SfaNI 1210 GATGC GCATC(5/9) SfoI 207 GGCGCC GGC^GCC Sse8387I 1230 CCTGCAGG CCTGCA^GG XhoII 483 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 121 GAATTC [GA]^AATT[CT] 498 GAATTC Alw44I 282 GTGCAC G^TGCAC 974 GTGCAC AlwI 484 GATCC GGATC(4/5) 449 GGATC ApaLI 282 GTGCAC G^TGCAC 974 GTGCAC ApoI 121 GAATTC [GA]^AATT[CT] 498 GAATTC AspHI 282 GTGCAC G[AT]GC[AT]^C 974 GTGCAC BbrPI 355 CACGTG CAC^GTG 1021 CACGTG BsaAI 355 CACGTG [CT]AC^GT[GA] 1021 CACGTG BsiEI 227 CGGCCG CG[GA][CT]^CG 1047 CGGCCG BsiHKAI 282 GTGCAC G[AT]GC[AT]^C 974 GTGCAC BsmAI 1120 GAGAC GTCTC(1/5) 519 GTCTC BsrFI 36 GCCGGC [GA]^CCGG[CT] 949 GCCGGC Cfr10I 36 GCCGGC [GA]^CCGG[CT] 949 GCCGGC EagI 227 CGGCCG C^GGCCG 1047 CGGCCG EclXI 227 CGGCCG C^GGCCG 1047 CGGCCG Eco52I 227 CGGCCG C^GGCCG 1047 CGGCCG EcoRI 121 GAATTC G^AATTC 498 GAATTC EcoT14I 140 CCAAGG C^C[AT][AT]GG 848 CCAAGG HgaI 79 GACGC GACGC(5/10) 946 GACGC NaeI 36 GCCGGC GCC^GGC 949 GCCGGC NgoMIV 36 GCCGGC G^CCGGC 949 GCCGGC NlaIII 393 CATG CATG^ 399 CATG PmaCI 355 CACGTG CAC^GTG 1021 CACGTG PmlI 355 CACGTG CAC^GTG 1021 CACGTG PpuMI 214 GGGTCCC [GA]G^G[AT]CC[CT] 851 AGGTCCC SfiI 396 GGCCATGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 405 GGCCAGCAAGGCC StyI 140 CCAAGG C^C[AT][AT]GG 848 CCAAGG Tsp509I 122 AATT ^AATT 499 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfcI SmaI TaqI TseI TspRI TthHB8I XmaCI XmaI