Restriction map for
pF1KB7721
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspEI BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 314 CGCG CG^CG
AlwI 1071 GATCC GGATC(4/5)
AlwNI 1057 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1198 CCTGAGG CC^T[ACGT]AGG
BalI 2 TGGCCA TGG^CCA
BanI 1224 GGCACC G^G[CT][GA]CC
BanII 295 GGGCTC G[GA]GC[CT]^C
BbrPI 747 CACGTG CAC^GTG
BbsI 139 GTCTTC GAAGAC(2/6)
BbvCI 810 CCTCAGC CCTCAGC(-5/-2)
BfaI 1118 CTAG C^TAG
BglII 362 AGATCT A^GATCT
BpuAI 139 GTCTTC GAAGAC(2/6)
BsaAI 747 CACGTG [CT]AC^GT[GA]
BsaBI 453 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 397 CTGCAC GTGCAG(16/14)
BsmI 832 GAATGC GAATGC(1/-1)
BspDI 791 ATCGAT AT^CGAT
BsrFI 711 GCCGGT [GA]^CCGG[CT]
BssSI 871 CTCGTG CACGAG(-5/-1)
BstUI 314 CGCG CG^CG
BstXI 5 CCAAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1198 CCTGAGG CC^T[ACGT]AGG
CfoI 326 GCGC GCG^C
Cfr10I 711 GCCGGT [GA]^CCGG[CT]
ClaI 791 ATCGAT AT^CGAT
DraII 1194 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1115 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 946 GAAGAG CTCTTC(1/4)
Eco81I 1198 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1194 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 516 CCATGG C^C[AT][AT]GG
HhaI 326 GCGC GCG^C
HinP1I 326 GCGC G^CGC
HincII 439 GTCAAC GT[CT]^[GA]AC
HindII 439 GTCAAC GT[CT]^[GA]AC
HindIII 536 AAGCTT A^AGCTT
Ksp632I 946 GAAGAG CTCTTC(1/4)
MaeI 1118 CTAG C^TAG
MamI 453 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MvnI 314 CGCG CG^CG
MwoI 272 GCGATGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 516 CCATGG C^CATGG
NdeI 923 CATATG CA^TATG
PmaCI 747 CACGTG CAC^GTG
PmlI 747 CACGTG CAC^GTG
PpuMI 1194 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 349 CAGCTG CAG^CTG
ScaI 989 AGTACT AGT^ACT
SexAI 722 ACCAGGT A^CC[AT]GGT
SmaI 292 CCCGGG CCC^GGG
SmlI 472 CTCAAG C^T[CT][GA]AG
StyI 516 CCATGG C^C[AT][AT]GG
Tsp509I 589 AATT ^AATT
XcmI 48 CCACTACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 292 CCCGGG C^CCGGG
XmaI 292 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 477 GTAC GT^AC
990 GTAC
AflIII 170 ACATGT A^C[GA][CT]GT
614 ACATGT
AspHI 490 GTGCTC G[AT]GC[AT]^C
757 GTGCTC
AspI 553 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
564 GACGGTGTC
AvaI 292 CCCGGG C^[CT]CG[GA]G
485 CTCGGG
BpmI 760 CTCCAG CTGGAG(16/14)
463 CTGGAG
BsaI 1247 GAGACC GGTCTC(1/5)
118 GGTCTC
BsiHKAI 490 GTGCTC G[AT]GC[AT]^C
757 GTGCTC
BsoBI 292 CCCGGG C^[CT]CG[GA]G
485 CTCGGG
BspHI 560 TCATGA T^CATGA
1131 TCATGA
BspLU11I 170 ACATGT A^CATGT
614 ACATGT
BstYI 362 AGATCT [GA]^GATC[CT]
1070 AGATCC
DdeI 811 CTCAG C^T[ACGT]AG
1199 CTGAG
DsaI 11 CCGTGG C^C[GA][CT]GG
516 CCATGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
639 TGGCCG
EcoNI 601 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1157 CCTATGACAGG
MflI 362 AGATCT [GA]^GATC[CT]
1070 AGATCC
MspA1I 349 CAGCTG C[AC]G^C[GT]G
912 CCGCTG
NlaIV 1194 GGGTCC GG[ACGT]^[ACGT]CC
1224 GGCACC
NspI 170 ACATGT [GA]CATG^[CT]
614 ACATGT
PflFI 553 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
564 GACGGTGTC
PstI 208 CTGCAG CTGCA^G
1142 CTGCAG
RcaI 560 TCATGA T^CATGA
1131 TCATGA
RsaI 477 GTAC GT^AC
990 GTAC
StuI 155 AGGCCT AGG^CCT
419 AGGCCT
TfiI 1041 GAATC G^A[AT]TC
1128 GATTC
Tsp45I 521 GTGAC ^GT[GC]AC
611 GTGAC
Tth111I 553 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
564 GACGGTGTC
XhoII 362 AGATCT [GA]^GATC[CT]
1070 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaII BbvI BcgI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI NciI
NdeII NlaIII PflMI PleI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI TspRI TthHB8I Van91I