Restriction map for
pF1KB7758
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 760 GACGTC GACGT^C
AccI 19 GTAGAC GT^[AC][GT]AC
AcsI 996 GAATTC [GA]^AATT[CT]
AcyI 760 GACGTC G[GA]^CG[CT]C
AflIII 1243 ACATGT A^C[GA][CT]GT
ApoI 996 GAATTC [GA]^AATT[CT]
Asp700I 552 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 669 GGTCC G^G[AT]CC
BamHI 1100 GGATCC G^GATCC
BbsI 1030 GTCTTC GAAGAC(2/6)
BclI 140 TGATCA T^GATCA
BpuAI 1030 GTCTTC GAAGAC(2/6)
BsaHI 760 GACGTC G[GA]^CG[CT]C
BsgI 984 CTGCAC GTGCAG(16/14)
BsmFI 1465 GTCCC GGGAC(10/14)
BspHI 764 TCATGA T^CATGA
BspLU11I 1243 ACATGT A^CATGT
BstBI 933 TTCGAA TT^CGAA
BstXI 47 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 669 GGTCC G^G[ACGT]CC
DrdI 661 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 996 GAATTC G^AATTC
FbaI 140 TGATCA T^GATCA
Hin1I 760 GACGTC G[GA]^CG[CT]C
MslI 1470 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 297 CCATGG C^CATGG
NspI 1243 ACATGT [GA]CATG^[CT]
NspV 933 TTCGAA TT^CGAA
PshAI 879 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 764 TCATGA T^CATGA
SapI 1143 GAAGAGC GCTCTTC(1/4)
Sau96I 669 GGTCC G^G[ACGT]CC
ScaI 890 AGTACT AGT^ACT
SfuI 933 TTCGAA TT^CGAA
SmlI 147 CTCAAG C^T[CT][GA]AG
XmnI 552 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 96 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
445 CAGAGGCTG
BalI 90 TGGCCA TGG^CCA
221 TGGCCA
BstEII 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
BstPI 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
DsaI 297 CCATGG C^C[GA][CT]GG
1383 CCACGG
EaeI 90 TGGCCA [CT]^GGCC[GA]
221 TGGCCA
EcoO65I 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
HaeIII 91 GGCC GG^CC
222 GGCC
MaeII 761 ACGT A^CGT
905 ACGT
MluNI 90 TGGCCA TGG^CCA
221 TGGCCA
MscI 90 TGGCCA TGG^CCA
221 TGGCCA
MseI 869 TTAA T^TAA
992 TTAA
NlaIV 197 GGAACC GG[ACGT]^[ACGT]CC
1100 GGATCC
PvuII 162 CAGCTG CAG^CTG
664 CAGCTG
SspI 1043 AATATT AAT^ATT
1061 AATATT
Tru9I 869 TTAA T^TAA
992 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbvI BfaI BglII BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MspA1I MvaI MwoI
NdeII NlaIII PleI PstI RsaI
SacI Sau3AI ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII