################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstNI BstPI BstUI BstXI Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Asp700I 138 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 335 GAGCAC G[AT]GC[AT]^C AspI 474 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 312 GCTGAGG CCTCAGC(-5/-2) BcnI 432 CCGGG CC^[GC]GG BfaI 403 CTAG C^TAG BglII 219 AGATCT A^GATCT BmyI 335 GAGCAC G[AGT]GC[ACT]^C BpmI 343 CTGGAG CTGGAG(16/14) BsaBI 64 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 335 GAGCAC G[AT]GC[AT]^C BsmFI 457 GTCCC GGGAC(10/14) Bsp1286I 335 GAGCAC G[AGT]GC[ACT]^C BspMI 420 GCAGGT ACCTGC(4/8) BsrI 107 CCAGT ACTGG(1/-1) BssKI 432 CCGGG ^CC[ACGT]GG BstYI 219 AGATCT [GA]^GATC[CT] Cac8I 333 GCGAGC GC[ACGT]^[ACGT]GC CfoI 7 GCGC GCG^C DraIII 430 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 289 CTCTTC CTCTTC(1/4) HaeII 6 GGCGCT [GA]GCGC^[CT] HapII 432 CCGG C^CGG HhaI 7 GCGC GCG^C HinP1I 7 GCGC G^CGC HpaII 432 CCGG C^CGG Ksp632I 289 CTCTTC CTCTTC(1/4) MaeI 403 CTAG C^TAG MaeIII 519 GTCAC ^GT[ACGT]AC MamI 64 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 219 AGATCT [GA]^GATC[CT] MseI 442 TTAA T^TAA MspI 432 CCGG C^CGG MwoI 333 GCGAGCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 432 CCGGG CC^[GC]GG NlaIII 351 CATG CATG^ NlaIV 18 GGAACC GG[ACGT]^[ACGT]CC PflFI 474 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 417 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 295 GACTC GAGTC(4/5) PstI 490 CTGCAG CTGCA^G ScaI 86 AGTACT AGT^ACT ScrFI 432 CCGGG CC^[ACGT]GG SfcI 490 CTGCAG C^T[GA][CT]AG TfiI 51 GATTC G^A[AT]TC Tru9I 442 TTAA T^TAA Tsp45I 519 GTCAC ^GT[GC]AC Tth111I 474 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 417 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 44 CCATCCTGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 219 AGATCT [GA]^GATC[CT] XmnI 138 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 30 GTATAC GT^[AC][GT]AC 202 GTATAC AfaI 87 GTAC GT^AC 427 GTAC AluI 244 AGCT AG^CT 260 AGCT AlwI 399 GATCC GGATC(4/5) 412 GATCC AlwNI 307 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 484 CAGGTGCTG BbvI 242 GCAGC GCAGC(8/12) 489 GCTGC BsmAI 287 GTCTC GTCTC(1/5) 480 GTCTC Bst1107I 30 GTATAC GTA^TAC 202 GTATAC BstF5I 45 CATCC GGATG(2/0) 375 GGATG BstZ17I 30 GTATAC GTA^TAC 202 GTATAC DdeI 313 CTGAG C^T[ACGT]AG 482 CTCAG Fnu4HI 242 GCAGC GC^[ACGT]GC 489 GCTGC FokI 45 CATCC GGATG(9/13) 375 GGATG HaeIII 126 GGCC GG^CC 190 GGCC HinfI 51 GATTC G^A[ACGT]TC 295 GACTC HphI 396 GGTGA GGTGA(8/7) 448 GGTGA ItaI 242 GCAGC GC^[ACGT]GC 489 GCTGC RsaI 87 GTAC GT^AC 427 GTAC SfaNI 264 GATGC GCATC(5/9) 309 GATGC StuI 125 AGGCCT AGG^CCT 189 AGGCCT TaqI 72 TCGA T^CGA 293 TCGA TseI 242 GCAGC G^C[AT]GC 489 GCTGC Tsp509I 174 AATT ^AATT 381 AATT TthHB8I 72 TCGA T^CGA 293 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### BsiYI BslI DpnII MboI MboII MnlI NdeII Sau3AI TspRI