Restriction map for
pF1KB8108
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BcgI BclI BfaI
BfrI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 1155 AAATTT [GA]^AATT[CT]
AfaI 1103 GTAC GT^AC
ApoI 1155 AAATTT [GA]^AATT[CT]
AvaII 322 GGACC G^G[AT]CC
BamHI 54 GGATCC G^GATCC
BbvCI 91 GCTGAGG CCTCAGC(-5/-2)
BciVI 369 GTATCC GTATCC(6/5)
BcnI 29 CCGGG CC^[GC]GG
BsaAI 544 TACGTA [CT]AC^GT[GA]
BsaJI 565 CCTGGG C^C[ACGT][ACGT]GG
BsiYI 700 CCCAATGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 700 CCCAATGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 400 GAATGC GAATGC(1/-1)
BspHI 1122 TCATGA T^CATGA
BsrFI 706 GCCGGT [GA]^CCGG[CT]
BstXI 36 CCACCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 643 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 1366 GCGC GCG^C
Cfr10I 706 GCCGGT [GA]^CCGG[CT]
DraII 138 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 138 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 4 GCGGG CCCGC(4/6)
HgaI 1392 GCGTC GACGC(5/10)
HhaI 1366 GCGC GCG^C
HinP1I 1366 GCGC G^CGC
MspA1I 434 CAGCTG C[AC]G^C[GT]G
NciI 29 CCGGG CC^[GC]GG
PleI 925 GAGTC GAGTC(4/5)
PvuII 434 CAGCTG CAG^CTG
RcaI 1122 TCATGA T^CATGA
RsaI 1103 GTAC GT^AC
SapI 129 GAAGAGC GCTCTTC(1/4)
SfaNI 844 GATGC GCATC(5/9)
SnaBI 544 TACGTA TAC^GTA
StuI 645 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 1170 ACATGT A^C[GA][CT]GT
1399 ACATGT
BbsI 601 GAAGAC GAAGAC(2/6)
951 GAAGAC
BglI 82 GCCTCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1176 GCCAATAAGGC
BpuAI 601 GAAGAC GAAGAC(2/6)
951 GAAGAC
BsaBI 579 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1324 GATCCATATC
BseRI 13 GAGGAG GAGGAG(10/8)
1421 GAGGAG
BspLU11I 1170 ACATGT A^CATGT
1399 ACATGT
BstYI 54 GGATCC [GA]^GATC[CT]
1323 AGATCC
Cfr13I 139 GGGCC G^G[ACGT]CC
322 GGACC
HapII 29 CCGG C^CGG
707 CCGG
HpaII 29 CCGG C^CGG
707 CCGG
HphI 709 GGTGA GGTGA(8/7)
901 GGTGA
MaeII 545 ACGT A^CGT
823 ACGT
MamI 579 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1324 GATCCATATC
MfeI 995 CAATTG C^AATTG
1085 CAATTG
MflI 54 GGATCC [GA]^GATC[CT]
1323 AGATCC
MspI 29 CCGG C^CGG
707 CCGG
MunI 995 CAATTG C^AATTG
1085 CAATTG
PstI 146 CTGCAG CTGCA^G
515 CTGCAG
Sau96I 139 GGGCC G^G[ACGT]CC
322 GGACC
SfcI 146 CTGCAG C^T[GA][CT]AG
515 CTGCAG
TaqI 507 TCGA T^CGA
528 TCGA
TfiI 175 GAATC G^A[AT]TC
1299 GAATC
TthHB8I 507 TCGA T^CGA
528 TCGA
XhoII 54 GGATCC [GA]^GATC[CT]
1323 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AlwI BbvI BsrI
BssKI BstF5I BstNI DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI ScrFI Tru9I TseI Tsp45I
Tsp509I TspRI