Restriction map for
pF1KB8113
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FbaI FseI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 94 GTCTAC GT^[AC][GT]AC
AccII 762 CGCG CG^CG
AflIII 90 ACGTGT A^C[GA][CT]GT
AlwNI 937 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 971 TGCGCA TGC^GCA
BamHI 848 GGATCC G^GATCC
BanI 231 GGCACC G^G[CT][GA]CC
BglI 445 GCCGGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 111 AGATCT A^GATCT
BsaAI 175 TACGTG [CT]AC^GT[GA]
BsmAI 383 GTCTC GTCTC(1/5)
BsrBI 318 GAGCGG CCGCTC(-3/-3)
BstUI 762 CGCG CG^CG
BstXI 284 CCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 972 GCGC GCG^C
DraIII 634 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 506 GATATC GAT^ATC
EcoT14I 248 CCTTGG C^C[AT][AT]GG
FspI 971 TGCGCA TGC^GCA
HhaI 972 GCGC GCG^C
HinP1I 972 GCGC G^CGC
HphI 821 TCACC GGTGA(8/7)
MaeIII 1022 GTGAC ^GT[ACGT]AC
MseI 755 TTAA T^TAA
MslI 861 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 762 CGCG CG^CG
PflMI 633 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1061 CTGCAG CTGCA^G
SmlI 388 CTCAAG C^T[CT][GA]AG
StyI 248 CCTTGG C^C[AT][AT]GG
TaqI 156 TCGA T^CGA
TfiI 416 GATTC G^A[AT]TC
Tru9I 755 TTAA T^TAA
Tsp45I 1022 GTGAC ^GT[GC]AC
TthHB8I 156 TCGA T^CGA
Van91I 633 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 72 TCTAGA T^CTAGA
XcmI 239 CCAACGCCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 849 GATCC GGATC(4/5)
848 GGATC
AvaII 372 GGACC G^G[AT]CC
572 GGTCC
BalI 641 TGGCCA TGG^CCA
1101 TGGCCA
BbsI 76 GAAGAC GAAGAC(2/6)
799 GTCTTC
BcnI 311 CCCGG CC^[GC]GG
1026 CCGGG
BfaI 73 CTAG C^TAG
1093 CTAG
BpuAI 76 GAAGAC GAAGAC(2/6)
799 GTCTTC
BstYI 111 AGATCT [GA]^GATC[CT]
848 GGATCC
DdeI 108 CTCAG C^T[ACGT]AG
1044 CTCAG
MaeI 73 CTAG C^TAG
1093 CTAG
MflI 111 AGATCT [GA]^GATC[CT]
848 GGATCC
MluNI 641 TGGCCA TGG^CCA
1101 TGGCCA
MscI 641 TGGCCA TGG^CCA
1101 TGGCCA
NaeI 445 GCCGGC GCC^GGC
1085 GCCGGC
NciI 311 CCCGG CC^[GC]GG
1026 CCGGG
NgoMIV 445 GCCGGC G^CCGGC
1085 GCCGGC
SacI 880 GAGCTC GAGCT^C
1041 GAGCTC
SapI 141 GCTCTTC GCTCTTC(1/4)
579 GCTCTTC
SfcI 934 CTACAG C^T[GA][CT]AG
1061 CTGCAG
Tsp509I 679 AATT ^AATT
916 AATT
XhoII 111 AGATCT [GA]^GATC[CT]
848 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DpnII EaeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
TseI TspRI