Restriction map for
pF1KB8115
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BpmI BsaAI BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FauI FbaI FseI
FspI HaeII HaeIII HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MamI MluI MluNI MscI MslI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 109 TCCGGA T^CCGGA
AlwI 1033 GATCC GGATC(4/5)
AspHI 808 GAGCTC G[AT]GC[AT]^C
AvrII 579 CCTAGG C^CTAGG
BcnI 1179 CCGGG CC^[GC]GG
BglII 744 AGATCT A^GATCT
BlnI 579 CCTAGG C^CTAGG
BlpI 595 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 595 GCTCAGC GC^T[ACGT]AGC
BsaWI 109 TCCGGA [AT]^CCGG[AT]
BseAI 109 TCCGGA T^CCGGA
BsiHKAI 808 GAGCTC G[AT]GC[AT]^C
BsmAI 300 GTCTC GTCTC(1/5)
BsmFI 306 GGGAC GGGAC(10/14)
BspEI 109 TCCGGA T^CCGGA
BspHI 267 TCATGA T^CATGA
BspMI 1042 ACCTGC ACCTGC(4/8)
BstAPI 1169 GCAATCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 178 CCAGG CC^[AT]GG
CelII 595 GCTCAGC GC^T[ACGT]AGC
DsaI 148 CCATGG C^C[GA][CT]GG
EarI 833 GAAGAG CTCTTC(1/4)
EcoRII 178 CCAGG ^CC[AT]GG
EcoRV 271 GATATC GAT^ATC
HgaI 711 GCGTC GACGC(5/10)
HincII 684 GTTAAC GT[CT]^[GA]AC
HindII 684 GTTAAC GT[CT]^[GA]AC
HpaI 684 GTTAAC GTT^AAC
Ksp632I 833 GAAGAG CTCTTC(1/4)
MfeI 1161 CAATTG C^AATTG
MroI 109 TCCGGA T^CCGGA
MspA1I 995 CAGCTG C[AC]G^C[GT]G
MunI 1161 CAATTG C^AATTG
MvaI 178 CCAGG CC^[AT]GG
NciI 1179 CCGGG CC^[GC]GG
NcoI 148 CCATGG C^CATGG
NheI 882 GCTAGC G^CTAGC
NlaIV 81 GGAACC GG[ACGT]^[ACGT]CC
PstI 984 CTGCAG CTGCA^G
PvuII 995 CAGCTG CAG^CTG
RcaI 267 TCATGA T^CATGA
SacI 808 GAGCTC GAGCT^C
TaqI 585 TCGA T^CGA
TfiI 963 GATTC G^A[AT]TC
Tsp45I 28 GTCAC ^GT[GC]AC
TthHB8I 585 TCGA T^CGA
XbaI 121 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 457 GTAC GT^AC
859 GTAC
Asp700I 763 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1012 GAAACGGTTC
BanII 174 GAGCCC G[GA]GC[CT]^C
808 GAGCTC
BbsI 137 GTCTTC GAAGAC(2/6)
1080 GTCTTC
BmyI 174 GAGCCC G[AGT]GC[ACT]^C
808 GAGCTC
BpuAI 137 GTCTTC GAAGAC(2/6)
1080 GTCTTC
BseRI 53 GAGGAG GAGGAG(10/8)
805 GAGGAG
BsiYI 46 CCAATCCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
821 CCTTGCAATGG
BslI 46 CCAATCCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
821 CCTTGCAATGG
Bsp1286I 174 GAGCCC G[AGT]GC[ACT]^C
808 GAGCTC
Bsp1407I 456 TGTACA T^GTACA
858 TGTACA
BsrDI 217 GCAATG GCAATG(2/0)
825 GCAATG
BsrGI 456 TGTACA T^GTACA
858 TGTACA
BsrI 492 ACTGG ACTGG(1/-1)
1036 CCAGT
BssKI 178 CCAGG ^CC[ACGT]GG
1179 CCGGG
BstYI 744 AGATCT [GA]^GATC[CT]
1032 AGATCC
Cac8I 661 GCAAGC GC[ACGT]^[ACGT]GC
882 GCTAGC
HapII 110 CCGG C^CGG
1179 CCGG
HpaII 110 CCGG C^CGG
1179 CCGG
MaeII 534 ACGT A^CGT
618 ACGT
MflI 744 AGATCT [GA]^GATC[CT]
1032 AGATCC
MspI 110 CCGG C^CGG
1179 CCGG
NlaIII 149 CATG CATG^
268 CATG
RsaI 457 GTAC GT^AC
859 GTAC
ScrFI 178 CCAGG CC^[ACGT]GG
1179 CCGGG
SfaNI 724 GATGC GCATC(5/9)
129 GCATC
SfcI 421 CTACAG C^T[GA][CT]AG
984 CTGCAG
SmlI 397 CTCAAG C^T[CT][GA]AG
693 CTCAAG
SspBI 456 TGTACA T^GTACA
858 TGTACA
SspI 240 AATATT AAT^ATT
1114 AATATT
XhoII 744 AGATCT [GA]^GATC[CT]
1032 AGATCC
XmnI 763 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1012 GAAACGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI ApoI BbvI BfaI
BsaJI BstF5I DdeI DpnII EcoRI
EcoT14I Fnu4HI FokI HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MwoI NdeII PleI
Sau3AI StyI Tru9I TseI Tsp509I
TspRI