Restriction map for
pF1KB8126
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII AccI AccIII AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AvaI AviII AvrII
BalI BbeI BbrPI BbsI BcgI
BfaI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoRI EcoT22I FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KspI MaeI
MamI MluNI MroI MscI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1291 GGTACC G^GTACC
AflII 222 CTTAAG C^TTAAG
AflIII 502 ACGCGT A^C[GA][CT]GT
AlwNI 856 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1291 GGTACC G^GTACC
BamHI 1252 GGATCC G^GATCC
BanII 1498 GAGCCC G[GA]GC[CT]^C
BbvCI 258 GCTGAGG CCTCAGC(-5/-2)
BciVI 1208 GTATCC GTATCC(6/5)
BclI 923 TGATCA T^GATCA
BfrI 222 CTTAAG C^TTAAG
BsaI 60 GGTCTC GGTCTC(1/5)
BseRI 430 GAGGAG GAGGAG(10/8)
BsiEI 23 CGGCCG CG[GA][CT]^CG
BsmFI 139 GGGAC GGGAC(10/14)
BsmI 1524 GAATGC GAATGC(1/-1)
BsrDI 90 CATTGC GCAATG(2/0)
BssSI 1244 CTCGTG CACGAG(-5/-1)
BstEII 634 GGTGACC G^GT[ACGT]ACC
BstF5I 840 GGATG GGATG(2/0)
BstPI 634 GGTGACC G^GT[ACGT]ACC
BstXI 1295 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 989 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 23 CGGCCG [CT]^GGCC[GA]
EagI 23 CGGCCG C^GGCCG
EclXI 23 CGGCCG C^GGCCG
Eco52I 23 CGGCCG C^GGCCG
EcoO65I 634 GGTGACC G^GT[ACGT]ACC
EcoRV 1481 GATATC GAT^ATC
FbaI 923 TGATCA T^GATCA
FokI 840 GGATG GGATG(9/13)
HinfI 1383 GAGTC G^A[ACGT]TC
KpnI 1291 GGTACC GGTAC^C
MluI 502 ACGCGT A^CGCGT
MspA1I 1256 CCGCTG C[AC]G^C[GT]G
NspI 915 ACATGC [GA]CATG^[CT]
PleI 1383 GAGTC GAGTC(4/5)
SapI 166 GAAGAGC GCTCTTC(1/4)
SmlI 222 CTTAAG C^T[CT][GA]AG
StuI 1004 AGGCCT AGG^CCT
Tsp45I 635 GTGAC ^GT[GC]AC
XcmI 65 CCAGAAATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AclI 689 AACGTT AA^CGTT
1225 AACGTT
AcsI 545 GAATTT [GA]^AATT[CT]
1635 AAATTT
ApoI 545 GAATTT [GA]^AATT[CT]
1635 AAATTT
Asp700I 687 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1524 GAATGCTTTC
AvaII 30 GGTCC G^G[AT]CC
484 GGTCC
BanI 597 GGTGCC G^G[CT][GA]CC
1291 GGTACC
BcnI 529 CCCGG CC^[GC]GG
1037 CCCGG
BglII 1175 AGATCT A^GATCT
1592 AGATCT
BmyI 1498 GAGCCC G[AGT]GC[ACT]^C
1569 GGGCAC
Bsp1286I 1498 GAGCCC G[AGT]GC[ACT]^C
1569 GGGCAC
BspMI 1281 GCAGGT ACCTGC(4/8)
1288 GCAGGT
CfoI 12 GCGC GCG^C
35 GCGC
HgaI 501 GACGC GACGC(5/10)
1395 GCGTC
HhaI 12 GCGC GCG^C
35 GCGC
HinP1I 12 GCGC G^CGC
35 GCGC
MaeIII 635 GTGAC ^GT[ACGT]AC
1630 GTAAC
MslI 813 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
816 CATTGTGGTG
NciI 529 CCCGG CC^[GC]GG
1037 CCCGG
NcoI 884 CCATGG C^CATGG
1433 CCATGG
Psp1406I 689 AACGTT AA^CGTT
1225 AACGTT
TaqI 543 TCGA T^CGA
1358 TCGA
TthHB8I 543 TCGA T^CGA
1358 TCGA
XmnI 687 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1524 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BpmI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstNI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MfeI MflI MnlI MseI MspI
MunI MvaI MvnI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp509I TspRI XhoII