Restriction map for
pF1KB8128
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MaeII
MamI MflI MluI MluNI MroI
MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 553 GTAGAC GT^[AC][GT]AC
ApaI 111 GGGCCC GGGCC^C
Asp700I 497 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 740 GAGCTC G[AT]GC[AT]^C
AvaI 253 CTCGAG C^[CT]CG[GA]G
BclI 675 TGATCA T^GATCA
BsiHKAI 740 GAGCTC G[AT]GC[AT]^C
BsmAI 16 GTCTC GTCTC(1/5)
BsoBI 253 CTCGAG C^[CT]CG[GA]G
BstEII 208 GGTTACC G^GT[ACGT]ACC
BstNI 912 CCAGG CC^[AT]GG
BstPI 208 GGTTACC G^GT[ACGT]ACC
BstXI 235 CCAGTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 676 GATC ^GATC
DraII 111 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 828 CCATGG C^C[GA][CT]GG
EaeI 845 CGGCCA [CT]^GGCC[GA]
EarI 737 GAAGAG CTCTTC(1/4)
EcoO109I 111 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 208 GGTTACC G^GT[ACGT]ACC
EcoRII 912 CCAGG ^CC[AT]GG
FauI 109 GCGGG CCCGC(4/6)
FbaI 675 TGATCA T^GATCA
HaeII 319 GGCGCT [GA]GCGC^[CT]
HgaI 106 GACGC GACGC(5/10)
Ksp632I 737 GAAGAG CTCTTC(1/4)
MboI 676 GATC ^GATC
MfeI 295 CAATTG C^AATTG
MslI 770 CATACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 295 CAATTG C^AATTG
MvaI 912 CCAGG CC^[AT]GG
NcoI 828 CCATGG C^CATGG
NdeII 676 GATC ^GATC
PaeR7I 253 CTCGAG C^TCGAG
PleI 380 GAGTC GAGTC(4/5)
PstI 164 CTGCAG CTGCA^G
SacI 740 GAGCTC GAGCT^C
SapI 737 GAAGAGC GCTCTTC(1/4)
Sau3AI 676 GATC ^GATC
SfcI 164 CTGCAG C^T[GA][CT]AG
TaqI 254 TCGA T^CGA
TfiI 269 GATTC G^A[AT]TC
Tsp45I 413 GTGAC ^GT[GC]AC
TthHB8I 254 TCGA T^CGA
XcmI 539 CCATTGATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 253 CTCGAG C^TCGAG
XmnI 497 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 108 CGCG CG^CG
843 CGCG
AciI 109 GCGG CCGC(-3/-1)
844 GCGG
AvaII 246 GGACC G^G[AT]CC
519 GGACC
BanII 111 GGGCCC G[GA]GC[CT]^C
740 GAGCTC
BbvI 896 GCAGC GCAGC(8/12)
819 GCTGC
BciVI 231 GTATCC GTATCC(6/5)
723 GTATCC
BcnI 213 CCGGG CC^[GC]GG
513 CCCGG
BfaI 377 CTAG C^TAG
562 CTAG
BmyI 111 GGGCCC G[AGT]GC[ACT]^C
740 GAGCTC
BseRI 874 GAGGAG GAGGAG(10/8)
916 GAGGAG
BsmFI 245 GGGAC GGGAC(10/14)
644 GGGAC
Bsp1286I 111 GGGCCC G[AGT]GC[ACT]^C
740 GAGCTC
Bsp1407I 713 TGTACA T^GTACA
804 TGTACA
BsrGI 713 TGTACA T^GTACA
804 TGTACA
BstUI 108 CGCG CG^CG
843 CGCG
Cac8I 109 GCGGGC GC[ACGT]^[ACGT]GC
465 GCCTGC
CfoI 320 GCGC GCG^C
685 GCGC
EcoT14I 828 CCATGG C^C[AT][AT]GG
900 CCTTGG
HhaI 320 GCGC GCG^C
685 GCGC
HinP1I 320 GCGC G^CGC
685 GCGC
HinfI 269 GATTC G^A[ACGT]TC
380 GAGTC
MaeI 377 CTAG C^TAG
562 CTAG
MseI 11 TTAA T^TAA
56 TTAA
MvnI 108 CGCG CG^CG
843 CGCG
NciI 213 CCGGG CC^[GC]GG
513 CCCGG
NlaIII 549 CATG CATG^
829 CATG
SmlI 253 CTCGAG C^T[CT][GA]AG
880 CTTGAG
SspBI 713 TGTACA T^GTACA
804 TGTACA
StyI 828 CCATGG C^C[AT][AT]GG
900 CCTTGG
Tru9I 11 TTAA T^TAA
56 TTAA
TseI 819 GCTGC G^C[AT]GC
896 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsaJI
BsgI BsiYI BslI BsrI BssKI
BstF5I Cfr13I Fnu4HI FokI HaeIII
HapII HpaII ItaI MaeIII MboII
MnlI MspI MwoI NlaIV RsaI
Sau96I ScrFI SfaNI Tsp509I TspRI