Restriction map for
pF1KB8138
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII AclI AcsI AflII AgeI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I AspI AvrII BamHI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HinfI HpaI MaeI MfeI
MflI MluI MunI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp509I Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 893 GTCTAC GT^[AC][GT]AC
AccIII 624 TCCGGA T^CCGGA
AhdI 628 GACGCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 484 GTGCAC G^TGCAC
ApaI 1012 GGGCCC GGGCC^C
ApaLI 484 GTGCAC G^TGCAC
AspEI 628 GACGCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 484 GTGCAC G[AT]GC[AT]^C
AvaI 1371 CCCGAG C^[CT]CG[GA]G
BalI 282 TGGCCA TGG^CCA
BanII 1012 GGGCCC G[GA]GC[CT]^C
BbeI 1664 GGCGCC GGCGC^C
BbsI 475 GAAGAC GAAGAC(2/6)
BpmI 1276 CTGGAG CTGGAG(16/14)
BpuAI 475 GAAGAC GAAGAC(2/6)
BsaBI 280 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1497 GGTCTC GGTCTC(1/5)
BsaWI 624 TCCGGA [AT]^CCGG[AT]
BseAI 624 TCCGGA T^CCGGA
BsiHKAI 484 GTGCAC G[AT]GC[AT]^C
BsmBI 228 CGTCTC CGTCTC(1/5)
BsmI 394 GAATGC GAATGC(1/-1)
BsoBI 1371 CCCGAG C^[CT]CG[GA]G
BspEI 624 TCCGGA T^CCGGA
BsrFI 205 GCCGGC [GA]^CCGG[CT]
BstBI 391 TTCGAA TT^CGAA
BstXI 1514 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 205 GCCGGC [GA]^CCGG[CT]
Eam1105I 628 GACGCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1080 GAAGAG CTCTTC(1/4)
EcoNI 780 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 785 CCAAGG C^C[AT][AT]GG
HaeII 1664 GGCGCC [GA]GCGC^[CT]
KasI 1664 GGCGCC G^GCGCC
Ksp632I 1080 GAAGAG CTCTTC(1/4)
KspI 968 CCGCGG CCGC^GG
MamI 280 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 282 TGGCCA TGG^CCA
MroI 624 TCCGGA T^CCGGA
MscI 282 TGGCCA TGG^CCA
MseI 28 TTAA T^TAA
NaeI 205 GCCGGC GCC^GGC
NarI 1664 GGCGCC GG^CGCC
NgoMIV 205 GCCGGC G^CCGGC
NspI 271 GCATGC [GA]CATG^[CT]
NspV 391 TTCGAA TT^CGAA
PflMI 552 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1754 GGGACCC [GA]G^G[AT]CC[CT]
PstI 312 CTGCAG CTGCA^G
SacII 968 CCGCGG CCGC^GG
SfoI 1664 GGCGCC GGC^GCC
SfuI 391 TTCGAA TT^CGAA
SmlI 1764 CTTGAG C^T[CT][GA]AG
SphI 271 GCATGC GCATG^C
StyI 785 CCAAGG C^C[AT][AT]GG
Tru9I 28 TTAA T^TAA
Van91I 552 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 10 enzymes) #
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Acc65I 997 GGTACC G^GTACC
1705 GGTACC
AfaI 998 GTAC GT^AC
1706 GTAC
Asp718I 997 GGTACC G^GTACC
1705 GGTACC
BbvCI 1053 CCTCAGC CCTCAGC(-5/-2)
1234 GCTGAGG
BsgI 730 CTGCAC GTGCAG(16/14)
317 GTGCAG
EaeI 207 CGGCCA [CT]^GGCC[GA]
282 TGGCCA
KpnI 997 GGTACC GGTAC^C
1705 GGTACC
RsaI 998 GTAC GT^AC
1706 GTAC
SexAI 515 ACCTGGT A^CC[AT]GGT
1019 ACCAGGT
XcmI 1137 CCAACTCACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1343 CCAGCTGCCCGGTGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AlwI AlwNI AvaII AviII BanI
BbrPI BbvI BcnI BglI BmyI
BsaAI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PmaCI PmlI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I