Restriction map for
pF1KB8139
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BamHI BbeI
BbrPI BbvCI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 92 TCCGGA T^CCGGA
AflIII 449 ACGTGT A^C[GA][CT]GT
AlwI 65 GGATC GGATC(4/5)
AlwNI 118 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 112 GGGCCC GGGCC^C
AspI 19 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 61 CTCGGG C^[CT]CG[GA]G
BanI 812 GGCACC G^G[CT][GA]CC
BanII 112 GGGCCC G[GA]GC[CT]^C
BbsI 421 GTCTTC GAAGAC(2/6)
BfaI 292 CTAG C^TAG
BpuAI 421 GTCTTC GAAGAC(2/6)
BsaI 220 GAGACC GGTCTC(1/5)
BseAI 92 TCCGGA T^CCGGA
BsmBI 528 CGTCTC CGTCTC(1/5)
BsoBI 61 CTCGGG C^[CT]CG[GA]G
BspEI 92 TCCGGA T^CCGGA
BspMI 277 ACCTGC ACCTGC(4/8)
BstXI 816 CCACATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 65 GGATCT [GA]^GATC[CT]
EarI 48 GAAGAG CTCTTC(1/4)
HgaI 665 GCGTC GACGC(5/10)
Ksp632I 48 GAAGAG CTCTTC(1/4)
MaeI 292 CTAG C^TAG
MflI 65 GGATCT [GA]^GATC[CT]
MroI 92 TCCGGA T^CCGGA
MslI 783 CACGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 118 CAGCGG C[AC]G^C[GT]G
NlaIII 789 CATG CATG^
PflFI 19 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 117 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 352 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 469 CTGCAG CTGCA^G
SapI 48 GAAGAGC GCTCTTC(1/4)
SfcI 469 CTGCAG C^T[GA][CT]AG
TaqI 587 TCGA T^CGA
TfiI 914 GATTC G^A[AT]TC
Tsp509I 10 AATT ^AATT
Tth111I 19 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 587 TCGA T^CGA
Van91I 117 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 65 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 269 GTAC GT^AC
309 GTAC
AvaII 338 GGTCC G^G[AT]CC
708 GGTCC
BalI 239 TGGCCA TGG^CCA
683 TGGCCA
BcgI 785 CGACCATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
53 GCAGGAAGCTCG
BciVI 132 GTATCC GTATCC(6/5)
768 GTATCC
BcnI 159 CCGGG CC^[GC]GG
564 CCGGG
BmyI 112 GGGCCC G[AGT]GC[ACT]^C
750 GGGCAC
BsaAI 37 TACGTG [CT]AC^GT[GA]
266 CACGTA
BsaWI 71 ACCGGA [AT]^CCGG[AT]
92 TCCGGA
BseRI 904 CTCCTC GAGGAG(10/8)
301 GAGGAG
BsgI 774 CTGCAC GTGCAG(16/14)
391 GTGCAG
BsmAI 220 GAGAC GTCTC(1/5)
529 GTCTC
Bsp1286I 112 GGGCCC G[AGT]GC[ACT]^C
750 GGGCAC
BsrI 809 ACTGG ACTGG(1/-1)
823 ACTGG
BssSI 481 CACGAG CACGAG(-5/-1)
531 CTCGTG
DdeI 179 CTGAG C^T[ACGT]AG
285 CTCAG
EcoT14I 170 CCAAGG C^C[AT][AT]GG
215 CCAAGG
FauI 341 CCCGC CCCGC(4/6)
705 GCGGG
MluNI 239 TGGCCA TGG^CCA
683 TGGCCA
MscI 239 TGGCCA TGG^CCA
683 TGGCCA
NciI 159 CCGGG CC^[GC]GG
564 CCGGG
PpuMI 337 GGGTCCC [GA]G^G[AT]CC[CT]
707 GGGTCCT
RsaI 269 GTAC GT^AC
309 GTAC
SfaNI 568 GATGC GCATC(5/9)
607 GATGC
StyI 170 CCAAGG C^C[AT][AT]GG
215 CCAAGG
Tsp45I 17 GTGAC ^GT[GC]AC
175 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI BbvI BglI
BpmI BsaJI BsiYI BslI BsmFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspI MvaI MvnI
MwoI NdeII NlaIV PleI Sau3AI
Sau96I ScrFI TseI TspRI