################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII AccII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AspHI AspI AvaI AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI HindIII HpaI KasI KspI MamI MfeI MluI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 94 GTCTAC GT^[AC][GT]AC AccIII 353 TCCGGA T^CCGGA AflIII 947 ACGTGT A^C[GA][CT]GT Asp700I 255 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 149 TGGCCA TGG^CCA BfaI 73 CTAG C^TAG BsaWI 353 TCCGGA [AT]^CCGG[AT] BseAI 353 TCCGGA T^CCGGA BseRI 1051 GAGGAG GAGGAG(10/8) BsgI 943 CTGCAC GTGCAG(16/14) BsmBI 357 GAGACG CGTCTC(1/5) BsmFI 500 GGGAC GGGAC(10/14) BspEI 353 TCCGGA T^CCGGA BspHI 989 TCATGA T^CATGA BsrFI 194 GCCGGT [GA]^CCGG[CT] BssSI 466 CACGAG CACGAG(-5/-1) BstAPI 936 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 44 GGTTACC G^GT[ACGT]ACC BstPI 44 GGTTACC G^GT[ACGT]ACC BstYI 848 AGATCC [GA]^GATC[CT] Cfr10I 194 GCCGGT [GA]^CCGG[CT] DraIII 637 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 732 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 44 GGTTACC G^GT[ACGT]ACC EcoT14I 284 CCAAGG C^C[AT][AT]GG HaeII 135 GGCGCT [GA]GCGC^[CT] HincII 754 GTCAAC GT[CT]^[GA]AC HindII 754 GTCAAC GT[CT]^[GA]AC HphI 627 GGTGA GGTGA(8/7) MaeI 73 CTAG C^TAG MflI 848 AGATCC [GA]^GATC[CT] MluNI 149 TGGCCA TGG^CCA MroI 353 TCCGGA T^CCGGA MscI 149 TGGCCA TGG^CCA PflMI 636 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 989 TCATGA T^CATGA SphI 974 GCATGC GCATG^C StuI 740 AGGCCT AGG^CCT StyI 284 CCAAGG C^C[AT][AT]GG TaqI 375 TCGA T^CGA Tsp45I 58 GTCAC ^GT[GC]AC TthHB8I 375 TCGA T^CGA Van91I 636 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 848 AGATCC [GA]^GATC[CT] XmnI 255 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 506 GGTACC G^GTACC 545 GGTACC AcyI 359 GACGCC G[GA]^CG[CT]C 1151 GGCGTC ApaI 888 GGGCCC GGGCC^C 1105 GGGCCC Asp718I 506 GGTACC G^GTACC 545 GGTACC AviII 686 TGCGCA TGC^GCA 971 TGCGCA BbrPI 60 CACGTG CAC^GTG 946 CACGTG BbsI 76 GAAGAC GAAGAC(2/6) 811 GTCTTC BpuAI 76 GAAGAC GAAGAC(2/6) 811 GTCTTC BsaAI 60 CACGTG [CT]AC^GT[GA] 946 CACGTG BsaHI 359 GACGCC G[GA]^CG[CT]C 1151 GGCGTC BsmAI 357 GAGAC GTCTC(1/5) 300 GTCTC DdeI 190 CTGAG C^T[ACGT]AG 1133 CTCAG EarI 421 CTCTTC CTCTTC(1/4) 790 CTCTTC FspI 686 TGCGCA TGC^GCA 971 TGCGCA Hin1I 359 GACGCC G[GA]^CG[CT]C 1151 GGCGTC KpnI 506 GGTACC GGTAC^C 545 GGTACC Ksp632I 421 CTCTTC CTCTTC(1/4) 790 CTCTTC MaeII 61 ACGT A^CGT 947 ACGT MslI 473 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 651 CATCCTGATG NspI 761 ACATGC [GA]CATG^[CT] 974 GCATGC PmaCI 60 CACGTG CAC^GTG 946 CACGTG PmlI 60 CACGTG CAC^GTG 946 CACGTG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI TfiI TseI TspRI XcmI