Restriction map for
pF1KB8143
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AspI AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BseRI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI Ksp632I
KspI MamI MboI MfeI MflI
MluI MluNI MscI MseI MunI
NcoI NdeI NdeII NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
Sau3AI SbfI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 178 GGTACC G^GTACC
AccIII 426 TCCGGA T^CCGGA
AflIII 479 ACGTGT A^C[GA][CT]GT
ApaI 862 GGGCCC GGGCC^C
Asp718I 178 GGTACC G^GTACC
AviII 187 TGCGCA TGC^GCA
BbsI 1114 GTCTTC GAAGAC(2/6)
BfaI 215 CTAG C^TAG
BlpI 720 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 720 GCTGAGC GC^T[ACGT]AGC
BpuAI 1114 GTCTTC GAAGAC(2/6)
BsaI 646 GAGACC GGTCTC(1/5)
BseAI 426 TCCGGA T^CCGGA
BsgI 1093 CTGCAC GTGCAG(16/14)
BsmBI 577 GAGACG CGTCTC(1/5)
BspEI 426 TCCGGA T^CCGGA
BspMI 268 ACCTGC ACCTGC(4/8)
BsrBI 703 GAGCGG CCGCTC(-3/-3)
BstAPI 984 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 566 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 720 GCTGAGC GC^T[ACGT]AGC
DsaI 401 CCACGG C^C[GA][CT]GG
FspI 187 TGCGCA TGC^GCA
HincII 1150 GTCAAC GT[CT]^[GA]AC
HindII 1150 GTCAAC GT[CT]^[GA]AC
KpnI 178 GGTACC GGTAC^C
MaeI 215 CTAG C^TAG
MroI 426 TCCGGA T^CCGGA
MslI 267 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 13 GCCGGC GCC^GGC
NgoMIV 13 GCCGGC G^CCGGC
NotI 774 GCGGCCGC GC^GGCCGC
NspI 659 ACATGC [GA]CATG^[CT]
PflMI 519 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 805 GAGCTC GAGCT^C
ScaI 854 AGTACT AGT^ACT
SexAI 221 ACCTGGT A^CC[AT]GGT
TfiI 430 GATTC G^A[AT]TC
Tsp45I 410 GTGAC ^GT[GC]AC
Van91I 519 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 179 GTAC GT^AC
855 GTAC
AlwNI 63 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
520 CAGAACCTG
AspHI 805 GAGCTC G[AT]GC[AT]^C
982 GAGCAC
BsaWI 167 TCCGGT [AT]^CCGG[AT]
426 TCCGGA
BsiHKAI 805 GAGCTC G[AT]GC[AT]^C
982 GAGCAC
DraII 64 AGGGCCT [GA]G^G[ACGT]CC[CT]
894 AGGCCCC
EcoO109I 64 AGGGCCT [GA]G^G[ACGT]CC[CT]
894 AGGCCCC
EcoT14I 458 CCAAGG C^C[AT][AT]GG
698 CCAAGG
HphI 252 TCACC GGTGA(8/7)
266 TCACC
PleI 463 GACTC GAGTC(4/5)
1057 GACTC
RsaI 179 GTAC GT^AC
855 GTAC
SfaNI 771 GATGC GCATC(5/9)
206 GCATC
StyI 458 CCAAGG C^C[AT][AT]GG
698 CCAAGG
TaqI 320 TCGA T^CGA
1009 TCGA
TthHB8I 320 TCGA T^CGA
1009 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI Asp700I
AvaI AvaII BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI EaeI
EagI EclXI Eco52I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NlaIII
NlaIV PvuII Sau96I ScrFI SfoI
SmaI TseI TspRI XmaCI XmaI
XmnI