################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BmyI BpmI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CfoI Cfr10I Cfr13I CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII HphI KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 105 GTAC GT^AC BbsI 541 GAAGAC GAAGAC(2/6) BfaI 169 CTAG C^TAG BlpI 436 GCTGAGC GC^T[ACGT]AGC Bpu1102I 436 GCTGAGC GC^T[ACGT]AGC BpuAI 541 GAAGAC GAAGAC(2/6) BsaAI 457 TACGTG [CT]AC^GT[GA] BsaBI 189 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 17 CCAAGG C^C[ACGT][ACGT]GG BsiYI 249 CCGAAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 249 CCGAAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 236 GAGAC GTCTC(1/5) BspDI 190 ATCGAT AT^CGAT BsrI 369 ACTGG ACTGG(1/-1) BssSI 479 CACGAG CACGAG(-5/-1) CelII 436 GCTGAGC GC^T[ACGT]AGC ClaI 190 ATCGAT AT^CGAT DpnII 189 GATC ^GATC EcoRI 27 GAATTC G^AATTC EcoRV 193 GATATC GAT^ATC EcoT14I 17 CCAAGG C^C[AT][AT]GG HpaI 451 GTTAAC GTT^AAC MaeI 169 CTAG C^TAG MaeII 458 ACGT A^CGT MaeIII 377 GTAAC ^GT[ACGT]AC MamI 189 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 189 GATC ^GATC MslI 51 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 189 GATC ^GATC NlaIV 263 GGTTCC GG[ACGT]^[ACGT]CC PleI 90 GACTC GAGTC(4/5) RsaI 105 GTAC GT^AC Sau3AI 189 GATC ^GATC SfaNI 605 GATGC GCATC(5/9) StyI 17 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 27 GAATTC [GA]^AATT[CT] 521 AAATTC ApoI 27 GAATTC [GA]^AATT[CT] 521 AAATTC DdeI 198 CTTAG C^T[ACGT]AG 437 CTGAG EarI 418 GAAGAG CTCTTC(1/4) 570 GAAGAG HincII 451 GTTAAC GT[CT]^[GA]AC 490 GTTGAC HindII 451 GTTAAC GT[CT]^[GA]AC 490 GTTGAC Ksp632I 418 GAAGAG CTCTTC(1/4) 570 GAAGAG MspA1I 206 CAGCTG C[AC]G^C[GT]G 311 CAGCTG MwoI 62 GCAGTGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 304 GCAGCTACAGC NlaIII 54 CATG CATG^ 102 CATG PvuII 206 CAGCTG CAG^CTG 311 CAGCTG TaqI 191 TCGA T^CGA 525 TCGA TfiI 295 GAATC G^A[AT]TC 593 GAATC TspRI 63 CAGTG CA[GC]TG[ACGT][ACGT]^ 442 CAGTG TthHB8I 191 TCGA T^CGA 525 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BbvI Fnu4HI HinfI ItaI MboII MnlI MseI SfcI Tru9I TseI Tsp509I