Restriction map for
pF1KB8157
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AvaII AviII BamHI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BpmI BsaAI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KspI MaeII MfeI
MluI MroI MslI MspA1I MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 546 GGTACC G^GTACC
AlwI 454 GATCC GGATC(4/5)
AocI 475 CCTCAGG CC^T[ACGT]AGG
ApaI 1332 GGGCCC GGGCC^C
Asp718I 546 GGTACC G^GTACC
AspHI 158 GTGCTC G[AT]GC[AT]^C
AvrII 130 CCTAGG C^CTAGG
BalI 1343 TGGCCA TGG^CCA
BanII 1332 GGGCCC G[GA]GC[CT]^C
BbsI 1365 GTCTTC GAAGAC(2/6)
BciVI 850 GTATCC GTATCC(6/5)
BglII 540 AGATCT A^GATCT
BlnI 130 CCTAGG C^CTAGG
BlpI 190 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 190 GCTGAGC GC^T[ACGT]AGC
BpuAI 1365 GTCTTC GAAGAC(2/6)
BsaBI 900 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1369 TCCGGT [AT]^CCGG[AT]
BsiHKAI 158 GTGCTC G[AT]GC[AT]^C
BsmI 672 GAATGC GAATGC(1/-1)
BstAPI 35 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1125 CCAAAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 475 CCTCAGG CC^T[ACGT]AGG
CelII 190 GCTGAGC GC^T[ACGT]AGC
CfoI 955 GCGC GCG^C
DsaI 854 CCATGG C^C[GA][CT]GG
EaeI 1343 TGGCCA [CT]^GGCC[GA]
Eco81I 475 CCTCAGG CC^T[ACGT]AGG
EcoNI 1020 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 1370 CCGG C^CGG
HhaI 955 GCGC GCG^C
HinP1I 955 GCGC G^CGC
HincII 1568 GTCAAC GT[CT]^[GA]AC
HindII 1568 GTCAAC GT[CT]^[GA]AC
HpaII 1370 CCGG C^CGG
HphI 408 GGTGA GGTGA(8/7)
KpnI 546 GGTACC GGTAC^C
MamI 900 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1343 TGGCCA TGG^CCA
MscI 1343 TGGCCA TGG^CCA
MspI 1370 CCGG C^CGG
NcoI 854 CCATGG C^CATGG
PleI 1227 GACTC GAGTC(4/5)
PstI 1523 CTGCAG CTGCA^G
SapI 175 GCTCTTC GCTCTTC(1/4)
ScaI 1047 AGTACT AGT^ACT
SphI 872 GCATGC GCATG^C
StuI 7 AGGCCT AGG^CCT
TfiI 1136 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccI 1261 GTAGAC GT^[AC][GT]AC
1575 GTCTAC
AciI 51 GCGG CCGC(-3/-1)
1274 GCGG
BanI 546 GGTACC G^G[CT][GA]CC
1186 GGTGCC
BmyI 158 GTGCTC G[AGT]GC[ACT]^C
1332 GGGCCC
BsmAI 1531 GAGAC GTCTC(1/5)
1555 GAGAC
BsmFI 883 GTCCC GGGAC(10/14)
1063 GTCCC
Bsp1286I 158 GTGCTC G[AGT]GC[ACT]^C
1332 GGGCCC
BspMI 40 ACCTGC ACCTGC(4/8)
1564 GCAGGT
BstYI 453 AGATCC [GA]^GATC[CT]
540 AGATCT
EcoRI 372 GAATTC G^AATTC
1077 GAATTC
HinfI 1136 GAATC G^A[ACGT]TC
1227 GACTC
MaeIII 119 GTCAC ^GT[ACGT]AC
1469 GTGAC
MflI 453 AGATCC [GA]^GATC[CT]
540 AGATCT
NspI 872 GCATGC [GA]CATG^[CT]
1514 GCATGT
Tsp45I 119 GTCAC ^GT[GC]AC
1469 GTGAC
XhoII 453 AGATCC [GA]^GATC[CT]
540 AGATCT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BseRI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII ItaI Ksp632I MaeI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I