################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI Ksp632I KspI MamI MflI MluI MluNI MroI MscI MseI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 923 GACGTC GACGT^C Acc65I 425 GGTACC G^GTACC Aor51HI 110 AGCGCT AGC^GCT ApaI 27 GGGCCC GGGCC^C Asp700I 990 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 425 GGTACC G^GTACC AspHI 1036 GTGCTC G[AT]GC[AT]^C AvaI 1031 CTCGGG C^[CT]CG[GA]G BbeI 74 GGCGCC GGCGC^C BbsI 388 GTCTTC GAAGAC(2/6) BciVI 1008 GGATAC GTATCC(6/5) BpuAI 388 GTCTTC GAAGAC(2/6) BsgI 819 GTGCAG GTGCAG(16/14) BsiHKAI 1036 GTGCTC G[AT]GC[AT]^C BsmFI 656 GGGAC GGGAC(10/14) BsoBI 1031 CTCGGG C^[CT]CG[GA]G BspHI 617 TCATGA T^CATGA BstXI 1062 CCATGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 656 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 1062 CCATGG C^C[GA][CT]GG Eco47III 110 AGCGCT AGC^GCT EcoO109I 656 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 448 GATATC GAT^ATC FauI 35 CCCGC CCCGC(4/6) KasI 74 GGCGCC G^GCGCC KpnI 425 GGTACC GGTAC^C MfeI 567 CAATTG C^AATTG MslI 1063 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 567 CAATTG C^AATTG NarI 74 GGCGCC GG^CGCC NcoI 1062 CCATGG C^CATGG NspI 861 GCATGC [GA]CATG^[CT] PpuMI 656 GGGACCT [GA]G^G[AT]CC[CT] RcaI 617 TCATGA T^CATGA SfoI 74 GGCGCC GGC^GCC SphI 861 GCATGC GCATG^C Tsp509I 568 AATT ^AATT XcmI 337 CCACGACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 990 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcyI 74 GGCGCC G[GA]^CG[CT]C 923 GACGTC AlwNI 513 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 1112 CAGCCTCTG AvaII 252 GGTCC G^G[AT]CC 657 GGACC BanII 27 GGGCCC G[GA]GC[CT]^C 1084 GAGCCC BsaHI 74 GGCGCC G[GA]^CG[CT]C 923 GACGTC BsmBI 459 CGTCTC CGTCTC(1/5) 921 GAGACG BstAPI 125 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 512 GCAGCATCTGC BstF5I 315 CATCC GGATG(2/0) 782 GGATG DdeI 330 CTCAG C^T[ACGT]AG 1110 CTCAG EcoT14I 938 CCAAGG C^C[AT][AT]GG 1062 CCATGG FokI 315 CATCC GGATG(9/13) 782 GGATG Hin1I 74 GGCGCC G[GA]^CG[CT]C 923 GACGTC MaeII 221 ACGT A^CGT 924 ACGT PstI 510 CTGCAG CTGCA^G 639 CTGCAG StyI 938 CCAAGG C^C[AT][AT]GG 1062 CCATGG TfiI 372 GAATC G^A[AT]TC 957 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccI AciI AfaI AluI AlwI BanI BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I