Restriction map for
pF1KB8165
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI AspEI AspI
AvrII BbeI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MaeI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 535 GTCTAC GT^[AC][GT]AC
AccII 1468 CGCG CG^CG
Alw44I 208 GTGCAC G^TGCAC
AocI 379 CCTGAGG CC^T[ACGT]AGG
ApaI 180 GGGCCC GGGCC^C
ApaLI 208 GTGCAC G^TGCAC
Asp700I 789 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 923 CCCGAG C^[CT]CG[GA]G
AviII 302 TGCGCA TGC^GCA
BamHI 1355 GGATCC G^GATCC
BbrPI 146 CACGTG CAC^GTG
BbsI 1431 GTCTTC GAAGAC(2/6)
BbvCI 432 CCTCAGC CCTCAGC(-5/-2)
BglII 323 AGATCT A^GATCT
BpuAI 1431 GTCTTC GAAGAC(2/6)
BsaAI 146 CACGTG [CT]AC^GT[GA]
BsmBI 1150 GAGACG CGTCTC(1/5)
BsoBI 923 CCCGAG C^[CT]CG[GA]G
BspLU11I 1031 ACATGT A^CATGT
BspMI 872 ACCTGC ACCTGC(4/8)
BsrBI 1351 GAGCGG CCGCTC(-3/-3)
BssSI 211 CACGAG CACGAG(-5/-1)
BstUI 1468 CGCG CG^CG
BstXI 956 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 379 CCTGAGG CC^T[ACGT]AGG
DraIII 781 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 601 GAAGAG CTCTTC(1/4)
Eco81I 379 CCTGAGG CC^T[ACGT]AGG
FspI 302 TGCGCA TGC^GCA
HaeII 347 AGCGCC [GA]GCGC^[CT]
Ksp632I 601 GAAGAG CTCTTC(1/4)
MseI 497 TTAA T^TAA
MslI 771 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1468 CGCG CG^CG
PleI 1447 GACTC GAGTC(4/5)
PmaCI 146 CACGTG CAC^GTG
PmlI 146 CACGTG CAC^GTG
SacI 895 GAGCTC GAGCT^C
StuI 93 AGGCCT AGG^CCT
TfiI 1401 GATTC G^A[AT]TC
Tru9I 497 TTAA T^TAA
XcmI 804 CCAGCTGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 789 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 520 GGTACC G^GTACC
977 GGTACC
AflIII 145 ACACGT A^C[GA][CT]GT
1031 ACATGT
AlwNI 745 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
868 CAGAACCTG
Asp718I 520 GGTACC G^GTACC
977 GGTACC
AspHI 208 GTGCAC G[AT]GC[AT]^C
895 GAGCTC
BalI 1064 TGGCCA TGG^CCA
1156 TGGCCA
BcnI 627 CCGGG CC^[GC]GG
1087 CCGGG
BsaI 487 GAGACC GGTCTC(1/5)
854 GAGACC
BsiHKAI 208 GTGCAC G[AT]GC[AT]^C
895 GAGCTC
BsrFI 833 ACCGGC [GA]^CCGG[CT]
980 ACCGGC
BstAPI 837 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1309 GCAGAGGATGC
CfoI 303 GCGC GCG^C
348 GCGC
Cfr10I 833 ACCGGC [GA]^CCGG[CT]
980 ACCGGC
HhaI 303 GCGC GCG^C
348 GCGC
HinP1I 303 GCGC G^CGC
348 GCGC
HinfI 1401 GATTC G^A[ACGT]TC
1447 GACTC
KpnI 520 GGTACC GGTAC^C
977 GGTACC
MluNI 1064 TGGCCA TGG^CCA
1156 TGGCCA
MscI 1064 TGGCCA TGG^CCA
1156 TGGCCA
NciI 627 CCGGG CC^[GC]GG
1087 CCGGG
NspI 776 ACATGC [GA]CATG^[CT]
1031 ACATGT
PpuMI 647 AGGACCT [GA]G^G[AT]CC[CT]
734 AGGACCT
TaqI 1293 TCGA T^CGA
1438 TCGA
TthHB8I 1293 TCGA T^CGA
1438 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TseI Tsp45I TspRI XhoII