Restriction map for
pF1KB8170
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AgeI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbrPI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI StyI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 400 GAATTC [GA]^AATT[CT]
AcyI 176 GGCGCC G[GA]^CG[CT]C
AflIII 851 ACATGT A^C[GA][CT]GT
AhdI 37 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 593 AGCGCT AGC^GCT
ApoI 400 GAATTC [GA]^AATT[CT]
AspEI 37 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 278 GAGCTC G[AT]GC[AT]^C
AvaI 638 CCCGGG C^[CT]CG[GA]G
BamHI 431 GGATCC G^GATCC
BbeI 176 GGCGCC GGCGC^C
BbsI 162 GTCTTC GAAGAC(2/6)
BbvCI 1101 GCTGAGG CCTCAGC(-5/-2)
BcgI 110 GCATCGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 162 GTCTTC GAAGAC(2/6)
BsaHI 176 GGCGCC G[GA]^CG[CT]C
BsiHKAI 278 GAGCTC G[AT]GC[AT]^C
BsoBI 638 CCCGGG C^[CT]CG[GA]G
BspLU11I 851 ACATGT A^CATGT
BsrFI 173 GCCGGC [GA]^CCGG[CT]
BstXI 267 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 173 GCCGGC [GA]^CCGG[CT]
DraIII 1064 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 153 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 427 CCACGG C^C[GA][CT]GG
Eam1105I 37 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 593 AGCGCT AGC^GCT
EcoRI 400 GAATTC G^AATTC
HgaI 780 GACGC GACGC(5/10)
Hin1I 176 GGCGCC G[GA]^CG[CT]C
KasI 176 GGCGCC G^GCGCC
MaeII 422 ACGT A^CGT
MslI 78 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 173 GCCGGC GCC^GGC
NarI 176 GGCGCC GG^CGCC
NgoMIV 173 GCCGGC G^CCGGC
NspI 851 ACATGT [GA]CATG^[CT]
PvuII 139 CAGCTG CAG^CTG
SacI 278 GAGCTC GAGCT^C
SbfI 792 CCTGCAGG CCTGCA^GG
SfoI 176 GGCGCC GGC^GCC
SmaI 638 CCCGGG CCC^GGG
Sse8387I 792 CCTGCAGG CCTGCA^GG
SspI 814 AATATT AAT^ATT
StuI 669 AGGCCT AGG^CCT
Tsp45I 236 GTGAC ^GT[GC]AC
XmaCI 638 CCCGGG C^CCGGG
XmaI 638 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanI 176 GGCGCC G^G[CT][GA]CC
772 GGTGCC
BsaI 151 GAGACC GGTCTC(1/5)
263 GAGACC
BsmAI 151 GAGAC GTCTC(1/5)
263 GAGAC
BsmFI 68 GTCCC GGGAC(10/14)
936 GTCCC
BspMI 611 ACCTGC ACCTGC(4/8)
1097 ACCTGC
BstAPI 1063 GCACTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1092 GCACGACCTGC
FauI 71 CCCGC CCCGC(4/6)
1000 CCCGC
MaeIII 236 GTGAC ^GT[ACGT]AC
525 GTTAC
MspA1I 139 CAGCTG C[AC]G^C[GT]G
644 CAGCGG
PleI 1009 GACTC GAGTC(4/5)
985 GAGTC
PpuMI 608 AGGACCT [GA]G^G[AT]CC[CT]
1022 AGGTCCT
PstI 136 CTGCAG CTGCA^G
793 CTGCAG
SfcI 136 CTGCAG C^T[GA][CT]AG
793 CTGCAG
SmlI 190 CTTGAG C^T[CT][GA]AG
517 CTTGAG
Tsp509I 401 AATT ^AATT
966 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AvaII BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI TspRI TthHB8I XhoII