Restriction map for
pF1KB8174
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BsaAI BsaHI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MfeI MflI
MluI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tru9I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 45 GGTACC G^GTACC
AccIII 454 TCCGGA T^CCGGA
Asp700I 967 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 45 GGTACC G^GTACC
AspI 913 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1554 GGACC G^G[AT]CC
BanI 45 GGTACC G^G[CT][GA]CC
BbsI 79 GAAGAC GAAGAC(2/6)
BfaI 1376 CTAG C^TAG
BlpI 1389 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1389 GCTGAGC GC^T[ACGT]AGC
BpuAI 79 GAAGAC GAAGAC(2/6)
BsaBI 612 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1323 GGTCTC GGTCTC(1/5)
BsaWI 454 TCCGGA [AT]^CCGG[AT]
BseAI 454 TCCGGA T^CCGGA
BsgI 574 GTGCAG GTGCAG(16/14)
BsmFI 22 GTCCC GGGAC(10/14)
BspEI 454 TCCGGA T^CCGGA
BspMI 499 ACCTGC ACCTGC(4/8)
CelII 1389 GCTGAGC GC^T[ACGT]AGC
CfoI 1384 GCGC GCG^C
DraII 1105 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1660 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1105 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 1383 GGCGCT [GA]GCGC^[CT]
HhaI 1384 GCGC GCG^C
HinP1I 1384 GCGC G^CGC
KpnI 45 GGTACC GGTAC^C
MaeI 1376 CTAG C^TAG
MaeII 320 ACGT A^CGT
MamI 612 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 454 TCCGGA T^CCGGA
PflFI 913 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 501 CTGCAG CTGCA^G
SapI 1302 GCTCTTC GCTCTTC(1/4)
SmlI 1140 CTTGAG C^T[CT][GA]AG
SspI 1231 AATATT AAT^ATT
TfiI 72 GAATC G^A[AT]TC
Tth111I 913 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1196 CCATCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 967 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AcsI 514 AAATTT [GA]^AATT[CT]
722 AAATTT
AlwNI 866 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1559 CAGAAGCTG
ApoI 514 AAATTT [GA]^AATT[CT]
722 AAATTT
BcnI 1109 CCCGG CC^[GC]GG
1551 CCCGG
BsmAI 1597 GAGAC GTCTC(1/5)
1324 GTCTC
Cac8I 379 GCTGGC GC[ACGT]^[ACGT]GC
1122 GCCAGC
EcoNI 49 CCTACTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1248 CCTCAGCCAGG
MslI 735 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1200 CACCCCAGTG
NciI 1109 CCCGG CC^[GC]GG
1551 CCCGG
SacI 542 GAGCTC GAGCT^C
1392 GAGCTC
SfcI 221 CTATAG C^T[GA][CT]AG
501 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BanII BbvCI BbvI BclI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I Tsp509I TspRI