Restriction map for
pF1KB8176
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HgaI HincII HindII
HindIII HpaI Ksp632I KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 380 GGTACC G^GTACC
AcyI 97 GGCGCC G[GA]^CG[CT]C
AfaI 381 GTAC GT^AC
Aor51HI 50 AGCGCT AGC^GCT
Asp718I 380 GGTACC G^GTACC
AvaII 167 GGTCC G^G[AT]CC
BalI 65 TGGCCA TGG^CCA
BbeI 97 GGCGCC GGCGC^C
BbvI 228 GCAGC GCAGC(8/12)
BfaI 188 CTAG C^TAG
BpmI 174 CTGGAG CTGGAG(16/14)
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BsaWI 377 TCCGGT [AT]^CCGG[AT]
BseRI 115 GAGGAG GAGGAG(10/8)
BsiYI 170 CCGCCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 170 CCGCCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 665 GAGAC GTCTC(1/5)
BsrDI 597 CATTGC GCAATG(2/0)
BstXI 284 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 271 AGATCC [GA]^GATC[CT]
Eco47III 50 AGCGCT AGC^GCT
EcoT14I 435 CCAAGG C^C[AT][AT]GG
HapII 378 CCGG C^CGG
Hin1I 97 GGCGCC G[GA]^CG[CT]C
HpaII 378 CCGG C^CGG
KasI 97 GGCGCC G^GCGCC
KpnI 380 GGTACC GGTAC^C
MaeI 188 CTAG C^TAG
MaeII 149 ACGT A^CGT
MaeIII 85 GTGAC ^GT[ACGT]AC
MflI 271 AGATCC [GA]^GATC[CT]
MluNI 65 TGGCCA TGG^CCA
MscI 65 TGGCCA TGG^CCA
MspA1I 229 CAGCTG C[AC]G^C[GT]G
MspI 378 CCGG C^CGG
NarI 97 GGCGCC GG^CGCC
PvuII 229 CAGCTG CAG^CTG
RsaI 381 GTAC GT^AC
SfoI 97 GGCGCC GGC^GCC
SspI 308 AATATT AAT^ATT
StyI 435 CCAAGG C^C[AT][AT]GG
TfiI 540 GATTC G^A[AT]TC
TseI 228 GCAGC G^C[AT]GC
Tsp45I 85 GTGAC ^GT[GC]AC
XhoII 271 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 18 GATCC GGATC(4/5)
272 GATCC
BanI 97 GGCGCC G^G[CT][GA]CC
380 GGTACC
BglI 28 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
37 GCCGAGCAGGC
BssKI 173 CCTGG ^CC[ACGT]GG
501 CCTGG
BstNI 173 CCTGG CC^[AT]GG
501 CCTGG
Cfr13I 159 GGGCC G^G[ACGT]CC
167 GGTCC
DsaI 134 CCGTGG C^C[GA][CT]GG
284 CCACGG
EaeI 35 TGGCCG [CT]^GGCC[GA]
65 TGGCCA
EcoRII 173 CCTGG ^CC[AT]GG
501 CCTGG
HaeII 50 AGCGCT [GA]GCGC^[CT]
97 GGCGCC
HphI 367 GGTGA GGTGA(8/7)
403 GGTGA
MslI 69 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
282 CACCACGGTG
MvaI 173 CCTGG CC^[AT]GG
501 CCTGG
PleI 625 GACTC GAGTC(4/5)
259 GAGTC
Sau96I 159 GGGCC G^G[ACGT]CC
167 GGTCC
ScrFI 173 CCTGG CC^[ACGT]GG
501 CCTGG
SfiI 27 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
36 GGCCGAGCAGGCC
TaqI 194 TCGA T^CGA
411 TCGA
Tsp509I 296 AATT ^AATT
430 AATT
TthHB8I 194 TCGA T^CGA
411 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbsI BpuAI BsaJI
BsgI BspMI Cac8I CfoI DdeI
DpnII Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI MboI MboII MnlI
MseI MwoI NdeII NlaIII NlaIV
Sau3AI SfaNI Tru9I TspRI