Restriction map for
pF1KB8179
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# NO CUT ENZYMES ( 113 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI Asp700I AspEI
AviII AvrII BamHI BanII BbeI
BbsI BcgI BclI BfrI BglI
BlnI BpuAI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
Ksp632I KspI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspI StuI SwaI Tsp509I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1155 GACGTC GACGT^C
Acc65I 1115 GGTACC G^GTACC
Alw44I 1050 GTGCAC G^TGCAC
ApaLI 1050 GTGCAC G^TGCAC
AseI 1228 ATTAAT AT^TAAT
AsnI 1228 ATTAAT AT^TAAT
Asp718I 1115 GGTACC G^GTACC
AspI 34 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1115 GGTACC G^G[CT][GA]CC
BbrPI 291 CACGTG CAC^GTG
BbvCI 768 CCTCAGC CCTCAGC(-5/-2)
BciVI 256 GTATCC GTATCC(6/5)
BsaI 919 GAGACC GGTCTC(1/5)
BsmBI 657 CGTCTC CGTCTC(1/5)
Bsp1407I 338 TGTACA T^GTACA
BsrBI 76 GAGCGG CCGCTC(-3/-3)
BsrFI 297 GCCGGC [GA]^CCGG[CT]
BsrGI 338 TGTACA T^GTACA
Cfr10I 297 GCCGGC [GA]^CCGG[CT]
DsaI 1262 CCATGG C^C[GA][CT]GG
EcoT14I 1262 CCATGG C^C[AT][AT]GG
KpnI 1115 GGTACC GGTAC^C
MboII 198 GAAGA GAAGA(8/7)
MseI 1229 TTAA T^TAA
MslI 247 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 297 GCCGGC GCC^GGC
NcoI 1262 CCATGG C^CATGG
NgoMIV 297 GCCGGC G^CCGGC
NspI 518 GCATGC [GA]CATG^[CT]
PflFI 34 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 882 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 291 CACGTG CAC^GTG
PmlI 291 CACGTG CAC^GTG
PshAI 631 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1228 ATTAAT AT^TAAT
SmaI 929 CCCGGG CCC^GGG
SphI 518 GCATGC GCATG^C
SspBI 338 TGTACA T^GTACA
StyI 1262 CCATGG C^C[AT][AT]GG
Tru9I 1229 TTAA T^TAA
Tth111I 34 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 882 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 929 CCCGGG C^CCGGG
XmaI 929 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 934 GACGCC G[GA]^CG[CT]C
1155 GACGTC
AlwI 477 GATCC GGATC(4/5)
819 GATCC
BfaI 592 CTAG C^TAG
1099 CTAG
BglII 422 AGATCT A^GATCT
911 AGATCT
BlpI 121 GCTGAGC GC^T[ACGT]AGC
522 GCTCAGC
Bpu1102I 121 GCTGAGC GC^T[ACGT]AGC
522 GCTCAGC
BsaAI 103 TACGTG [CT]AC^GT[GA]
291 CACGTG
BsaBI 486 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1017 GATGAACATC
BsaHI 934 GACGCC G[GA]^CG[CT]C
1155 GACGTC
BspMI 848 ACCTGC ACCTGC(4/8)
884 ACCTGC
CelII 121 GCTGAGC GC^T[ACGT]AGC
522 GCTCAGC
DraIII 100 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
1120 CACTCCGTG
HaeII 671 AGCGCC [GA]GCGC^[CT]
1002 GGCGCT
Hin1I 934 GACGCC G[GA]^CG[CT]C
1155 GACGTC
MaeI 592 CTAG C^TAG
1099 CTAG
MamI 486 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1017 GATGAACATC
PstI 43 CTGCAG CTGCA^G
481 CTGCAG
PvuII 112 CAGCTG CAG^CTG
757 CAGCTG
SfcI 43 CTGCAG C^T[GA][CT]AG
481 CTGCAG
Tsp45I 183 GTCAC ^GT[GC]AC
870 GTCAC
XcmI 164 CCACCCTCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
304 CCAGGCGTGGGGTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BalI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI TspRI TthHB8I XhoII