Restriction map for
pF1KB8183
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstNI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRII EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI MvaI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AfaI 252 GTAC GT^AC
AlwNI 262 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 45 GGGCCC GGGCC^C
AspHI 243 GAGCAC G[AT]GC[AT]^C
AspI 550 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 394 GAAGAC GAAGAC(2/6)
BpuAI 394 GAAGAC GAAGAC(2/6)
BsiHKAI 243 GAGCAC G[AT]GC[AT]^C
BsmAI 79 GAGAC GTCTC(1/5)
BspMI 210 GCAGGT ACCTGC(4/8)
BstAPI 261 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 543 GGTCACC G^GT[ACGT]ACC
BstF5I 218 CATCC GGATG(2/0)
BstPI 543 GGTCACC G^GT[ACGT]ACC
DpnII 330 GATC ^GATC
EaeI 59 CGGCCA [CT]^GGCC[GA]
EcoO65I 543 GGTCACC G^GT[ACGT]ACC
EcoT14I 221 CCAAGG C^C[AT][AT]GG
FauI 23 GCGGG CCCGC(4/6)
FokI 218 CATCC GGATG(9/13)
HphI 545 TCACC GGTGA(8/7)
MaeII 398 ACGT A^CGT
MaeIII 544 GTCAC ^GT[ACGT]AC
MboI 330 GATC ^GATC
NdeII 330 GATC ^GATC
NspI 257 ACATGC [GA]CATG^[CT]
PflFI 550 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 274 GAGTC GAGTC(4/5)
PpuMI 212 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 252 GTAC GT^AC
Sau3AI 330 GATC ^GATC
SfaNI 457 GCATC GCATC(5/9)
SmaI 48 CCCGGG CCC^GGG
StyI 221 CCAAGG C^C[AT][AT]GG
Tsp45I 544 GTCAC ^GT[GC]AC
Tth111I 550 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 48 CCCGGG C^CCGGG
XmaI 48 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 28 GGCGCC G[GA]^CG[CT]C
602 GGCGCC
AvaI 48 CCCGGG C^[CT]CG[GA]G
97 CCCGAG
BanI 28 GGCGCC G^G[CT][GA]CC
602 GGCGCC
BanII 45 GGGCCC G[GA]GC[CT]^C
93 GAGCCC
BbeI 28 GGCGCC GGCGC^C
602 GGCGCC
BsaHI 28 GGCGCC G[GA]^CG[CT]C
602 GGCGCC
BsoBI 48 CCCGGG C^[CT]CG[GA]G
97 CCCGAG
BsrI 581 CCAGT ACTGG(1/-1)
587 CCAGT
Cac8I 34 GCAGGC GC[ACGT]^[ACGT]GC
261 GCAGGC
DraII 44 AGGGCCC [GA]G^G[ACGT]CC[CT]
212 AGGTCCC
EcoO109I 44 AGGGCCC [GA]G^G[ACGT]CC[CT]
212 AGGTCCC
Hin1I 28 GGCGCC G[GA]^CG[CT]C
602 GGCGCC
KasI 28 GGCGCC G^GCGCC
602 GGCGCC
NarI 28 GGCGCC GG^CGCC
602 GGCGCC
SfoI 28 GGCGCC GGC^GCC
602 GGCGCC
TfiI 282 GATTC G^A[AT]TC
297 GAATC
TspRI 83 CAGTG CA[GC]TG[ACGT][ACGT]^
588 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AvaII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BssKI CfoI Cfr13I DdeI
EarI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MboII MnlI MspI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TseI