Restriction map for
pF1KB8185
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MseI MspA1I MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwNI 784 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 120 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 514 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 370 GCCCGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1158 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1342 CTGCAC GTGCAG(16/14)
BsiEI 651 CGGCCG CG[GA][CT]^CG
BspHI 863 TCATGA T^CATGA
BsrFI 557 GCCGGC [GA]^CCGG[CT]
CfoI 975 GCGC GCG^C
Cfr10I 557 GCCGGC [GA]^CCGG[CT]
DraIII 892 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 511 GACGACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 574 CCGTGG C^C[GA][CT]GG
EagI 651 CGGCCG C^GGCCG
EarI 853 CTCTTC CTCTTC(1/4)
EclXI 651 CGGCCG C^GGCCG
Eco52I 651 CGGCCG C^GGCCG
HaeII 974 AGCGCC [GA]GCGC^[CT]
HhaI 975 GCGC GCG^C
HinP1I 975 GCGC G^CGC
HindIII 121 AAGCTT A^AGCTT
Ksp632I 853 CTCTTC CTCTTC(1/4)
MamI 1158 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 522 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 557 GCCGGC GCC^GGC
NgoMIV 557 GCCGGC G^CCGGC
PflFI 514 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1219 CTGCAG CTGCA^G
RcaI 863 TCATGA T^CATGA
SfcI 1219 CTGCAG C^T[GA][CT]AG
TfiI 922 GAATC G^A[AT]TC
Tsp509I 415 AATT ^AATT
Tth111I 514 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 120 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BalI 800 TGGCCA TGG^CCA
1290 TGGCCA
BanI 560 GGCACC G^G[CT][GA]CC
733 GGCACC
BbsI 931 GAAGAC GAAGAC(2/6)
668 GTCTTC
BpuAI 931 GAAGAC GAAGAC(2/6)
668 GTCTTC
BsmAI 331 GAGAC GTCTC(1/5)
659 GTCTC
BsrDI 611 CATTGC GCAATG(2/0)
713 GCAATG
DraII 253 GGGTCCC [GA]G^G[ACGT]CC[CT]
1042 GGGACCC
EcoO109I 253 GGGTCCC [GA]G^G[ACGT]CC[CT]
1042 GGGACCC
FauI 1142 GCGGG CCCGC(4/6)
1299 GCGGG
HgaI 174 GACGC GACGC(5/10)
675 GCGTC
MluNI 800 TGGCCA TGG^CCA
1290 TGGCCA
MscI 800 TGGCCA TGG^CCA
1290 TGGCCA
PflMI 891 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1355 CCAAGGACTGG
PleI 436 GACTC GAGTC(4/5)
1330 GACTC
PpuMI 253 GGGTCCC [GA]G^G[AT]CC[CT]
1042 GGGACCC
SfaNI 950 GCATC GCATC(5/9)
1190 GCATC
Van91I 891 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1355 CCAAGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII