Restriction map for
pF1KB8191
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AvaI
AviII BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tru9I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1087 GTCTAC GT^[AC][GT]AC
AccII 727 CGCG CG^CG
Alw44I 1107 GTGCAC G^TGCAC
ApaLI 1107 GTGCAC G^TGCAC
Asp700I 1020 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 810 CCTAGG C^CTAGG
BalI 280 TGGCCA TGG^CCA
BcgI 1118 GCATGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 811 CTAG C^TAG
BglII 1022 AGATCT A^GATCT
BlnI 810 CCTAGG C^CTAGG
BpmI 151 CTGGAG CTGGAG(16/14)
BsaI 532 GAGACC GGTCTC(1/5)
BseRI 79 GAGGAG GAGGAG(10/8)
BsmI 837 GAATGC GAATGC(1/-1)
BsrBI 1142 GAGCGG CCGCTC(-3/-3)
BssSI 301 CACGAG CACGAG(-5/-1)
BstUI 727 CGCG CG^CG
DraIII 183 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 314 CCATGG C^C[GA][CT]GG
EagI 440 CGGCCG C^GGCCG
EarI 872 GAAGAG CTCTTC(1/4)
EclXI 440 CGGCCG C^GGCCG
Eco52I 440 CGGCCG C^GGCCG
FauI 499 CCCGC CCCGC(4/6)
Ksp632I 872 GAAGAG CTCTTC(1/4)
MaeI 811 CTAG C^TAG
MaeII 176 ACGT A^CGT
MluNI 280 TGGCCA TGG^CCA
MscI 280 TGGCCA TGG^CCA
MvnI 727 CGCG CG^CG
NaeI 684 GCCGGC GCC^GGC
NcoI 314 CCATGG C^CATGG
NgoMIV 684 GCCGGC G^CCGGC
PstI 661 CTGCAG CTGCA^G
PvuI 29 CGATCG CGAT^CG
PvuII 139 CAGCTG CAG^CTG
SmlI 124 CTCAAG C^T[CT][GA]AG
SspI 523 AATATT AAT^ATT
TaqI 509 TCGA T^CGA
Tsp509I 793 AATT ^AATT
TthHB8I 509 TCGA T^CGA
XmnI 1020 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 1149 GTAC GT^AC
1172 GTAC
AlwI 435 GATCC GGATC(4/5)
999 GATCC
AspHI 696 GTGCTC G[AT]GC[AT]^C
1107 GTGCAC
AspI 352 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1222 GACAAGGTC
BanI 417 GGTGCC G^G[CT][GA]CC
977 GGCACC
BbsI 129 GAAGAC GAAGAC(2/6)
907 GTCTTC
BcnI 438 CCCGG CC^[GC]GG
981 CCCGG
BglI 41 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1048 GCCCTCCTGGC
BpuAI 129 GAAGAC GAAGAC(2/6)
907 GTCTTC
BsgI 289 CTGCAC GTGCAG(16/14)
1192 CTGCAC
BsiEI 29 CGATCG CG[GA][CT]^CG
440 CGGCCG
BsiHKAI 696 GTGCTC G[AT]GC[AT]^C
1107 GTGCAC
BsrFI 218 GCCGGT [GA]^CCGG[CT]
684 GCCGGC
Cfr10I 218 GCCGGT [GA]^CCGG[CT]
684 GCCGGC
DdeI 553 CTGAG C^T[ACGT]AG
922 CTCAG
DraII 205 AGGTCCC [GA]G^G[ACGT]CC[CT]
1226 AGGTCCT
EcoO109I 205 AGGTCCC [GA]G^G[ACGT]CC[CT]
1226 AGGTCCT
EcoT14I 314 CCATGG C^C[AT][AT]GG
810 CCTAGG
HgaI 601 GACGC GACGC(5/10)
728 GCGTC
HinfI 366 GAATC G^A[ACGT]TC
627 GAATC
MspA1I 139 CAGCTG C[AC]G^C[GT]G
1257 CCGCTG
NciI 438 CCCGG CC^[GC]GG
981 CCCGG
PflFI 352 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1222 GACAAGGTC
PflMI 538 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
591 CCACAGCATGG
PpuMI 205 AGGTCCC [GA]G^G[AT]CC[CT]
1226 AGGTCCT
RsaI 1149 GTAC GT^AC
1172 GTAC
SfcI 465 CTACAG C^T[GA][CT]AG
661 CTGCAG
StyI 314 CCATGG C^C[AT][AT]GG
810 CCTAGG
TfiI 366 GAATC G^A[AT]TC
627 GAATC
Tth111I 352 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1222 GACAAGGTC
Van91I 538 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
591 CCACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI Aor51HI AvaII BanII
BbvI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DpnII EaeI
Eco47III EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI XhoII