Restriction map for
pF1KB8198
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
Acc65I AccI AccII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BseRI
BsgI BsiEI BsiHKAI BsiWI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MluI MluNI MscI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 265 GACGTC GACGT^C
AccIII 209 TCCGGA T^CCGGA
AcyI 265 GACGTC G[GA]^CG[CT]C
AfaI 96 GTAC GT^AC
AflIII 92 ACACGT A^C[GA][CT]GT
AlwI 138 GATCC GGATC(4/5)
AvaII 212 GGACC G^G[AT]CC
BanII 239 GGGCTC G[GA]GC[CT]^C
BcnI 234 CCGGG CC^[GC]GG
BglII 179 AGATCT A^GATCT
BmyI 239 GGGCTC G[AGT]GC[ACT]^C
BpmI 524 CTGGAG CTGGAG(16/14)
BsaAI 93 CACGTA [CT]AC^GT[GA]
BsaHI 265 GACGTC G[GA]^CG[CT]C
BsaWI 209 TCCGGA [AT]^CCGG[AT]
BseAI 209 TCCGGA T^CCGGA
BsmAI 445 GAGAC GTCTC(1/5)
BsmBI 445 GAGACG CGTCTC(1/5)
BsmFI 185 GGGAC GGGAC(10/14)
BsmI 459 GAATGC GAATGC(1/-1)
Bsp1286I 239 GGGCTC G[AGT]GC[ACT]^C
BspEI 209 TCCGGA T^CCGGA
BspHI 485 TCATGA T^CATGA
BsrBI 544 GAGCGG CCGCTC(-3/-3)
BsrFI 582 GCCGGC [GA]^CCGG[CT]
CfoI 452 GCGC GCG^C
Cfr10I 582 GCCGGC [GA]^CCGG[CT]
DdeI 48 CTCAG C^T[ACGT]AG
DraII 519 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 491 CCATGG C^C[GA][CT]GG
EcoO109I 519 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 193 GCGGG CCCGC(4/6)
HaeII 451 AGCGCC [GA]GCGC^[CT]
HhaI 452 GCGC GCG^C
Hin1I 265 GACGTC G[GA]^CG[CT]C
HinP1I 452 GCGC G^CGC
HphI 374 TCACC GGTGA(8/7)
MaeIII 268 GTCAC ^GT[ACGT]AC
MroI 209 TCCGGA T^CCGGA
MseI 26 TTAA T^TAA
MspA1I 191 CAGCGG C[AC]G^C[GT]G
NaeI 582 GCCGGC GCC^GGC
NciI 234 CCGGG CC^[GC]GG
NcoI 491 CCATGG C^CATGG
NgoMIV 582 GCCGGC G^CCGGC
PstI 307 CTGCAG CTGCA^G
RcaI 485 TCATGA T^CATGA
RsaI 96 GTAC GT^AC
SfcI 307 CTGCAG C^T[GA][CT]AG
SmlI 556 CTCAAG C^T[CT][GA]AG
TfiI 280 GAATC G^A[AT]TC
Tru9I 26 TTAA T^TAA
Tsp45I 268 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 301 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
530 CAGCCTCTG
BsiYI 435 CCCCTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
583 CCGGCTGGCGG
BslI 435 CCCCTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
583 CCGGCTGGCGG
BstAPI 59 GCAAGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
300 GCAGTGGCTGC
BstYI 137 AGATCC [GA]^GATC[CT]
179 AGATCT
EcoT14I 407 CCAAGG C^C[AT][AT]GG
491 CCATGG
MflI 137 AGATCC [GA]^GATC[CT]
179 AGATCT
MnlI 372 CCTC CCTC(7/6)
533 CCTC
NlaIV 206 GGTTCC GG[ACGT]^[ACGT]CC
519 GGGGCC
PleI 46 GACTC GAGTC(4/5)
421 GACTC
SfaNI 248 GCATC GCATC(5/9)
431 GCATC
StyI 407 CCAAGG C^C[AT][AT]GG
491 CCATGG
TspRI 270 CACTG CA[GC]TG[ACGT][ACGT]^
301 CAGTG
XcmI 377 CCACCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
380 CCAAGAAGGTGGTGG
XhoII 137 AGATCC [GA]^GATC[CT]
179 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeII
MboI MslI MspI MvaI MwoI
NdeII NlaIII Sau3AI Sau96I ScrFI
TaqI TseI TthHB8I