Restriction map for
pF1KB8204
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BalI BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI HincII HindII
HpaI KpnI MaeIII MamI MluI
MluNI MroI MscI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 81 GGCGCC G[GA]^CG[CT]C
AhdI 3 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 90 GGGCCC GGGCC^C
AspEI 3 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1118 GTGCTC G[AT]GC[AT]^C
AviII 1400 TGCGCA TGC^GCA
BamHI 756 GGATCC G^GATCC
BbeI 81 GGCGCC GGCGC^C
BbvCI 475 GCTGAGG CCTCAGC(-5/-2)
BfaI 766 CTAG C^TAG
BglI 92 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 603 AGATCT A^GATCT
BsaHI 81 GGCGCC G[GA]^CG[CT]C
BsaI 1202 GAGACC GGTCTC(1/5)
BsiHKAI 1118 GTGCTC G[AT]GC[AT]^C
BspMI 1226 ACCTGC ACCTGC(4/8)
BsrDI 495 CATTGC GCAATG(2/0)
EaeI 1097 CGGCCA [CT]^GGCC[GA]
Eam1105I 3 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 86 CCATGG C^C[AT][AT]GG
FspI 1400 TGCGCA TGC^GCA
Hin1I 81 GGCGCC G[GA]^CG[CT]C
HindIII 1191 AAGCTT A^AGCTT
KasI 81 GGCGCC G^GCGCC
KspI 146 CCGCGG CCGC^GG
MaeI 766 CTAG C^TAG
MaeII 1043 ACGT A^CGT
MfeI 974 CAATTG C^AATTG
MspA1I 146 CCGCGG C[AC]G^C[GT]G
MunI 974 CAATTG C^AATTG
NaeI 1273 GCCGGC GCC^GGC
NarI 81 GGCGCC GG^CGCC
NcoI 86 CCATGG C^CATGG
NgoMIV 1273 GCCGGC G^CCGGC
PstI 847 CTGCAG CTGCA^G
SacII 146 CCGCGG CCGC^GG
SfcI 847 CTGCAG C^T[GA][CT]AG
SfoI 81 GGCGCC GGC^GCC
StyI 86 CCATGG C^C[AT][AT]GG
TaqI 590 TCGA T^CGA
Tsp509I 975 AATT ^AATT
TthHB8I 590 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 34 GTAC GT^AC
108 GTAC
BanI 81 GGCGCC G^G[CT][GA]CC
222 GGTGCC
BlpI 800 GCTCAGC GC^T[ACGT]AGC
1188 GCTAAGC
Bpu1102I 800 GCTCAGC GC^T[ACGT]AGC
1188 GCTAAGC
BsgI 708 CTGCAC GTGCAG(16/14)
784 CTGCAC
BsmBI 661 GAGACG CGTCTC(1/5)
1141 GAGACG
BsrBI 798 CCGCTC CCGCTC(-3/-3)
1233 CCGCTC
BsrFI 219 GCCGGT [GA]^CCGG[CT]
1273 GCCGGC
CelII 800 GCTCAGC GC^T[ACGT]AGC
1188 GCTAAGC
Cfr10I 219 GCCGGT [GA]^CCGG[CT]
1273 GCCGGC
DrdI 970 GACCCAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1143 GACGATTGAGTC
HphI 678 GGTGA GGTGA(8/7)
1257 GGTGA
MseI 455 TTAA T^TAA
1063 TTAA
MslI 993 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1038 CATTGACGTG
PpuMI 692 GGGACCT [GA]G^G[AT]CC[CT]
1247 GGGACCT
RsaI 34 GTAC GT^AC
108 GTAC
SmlI 1165 CTCAAG C^T[CT][GA]AG
1252 CTCAAG
Tru9I 455 TTAA T^TAA
1063 TTAA
TspRI 249 CAGTG CA[GC]TG[ACGT][ACGT]^
1116 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SmaI TfiI TseI
XhoII XmaCI XmaI