Restriction map for
pF1KB8229
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MaeII MamI
MfeI MluI MluNI MscI MslI
MunI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI TfiI
Tsp509I TspRI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AocI 92 CCTCAGG CC^T[ACGT]AGG
Aor51HI 323 AGCGCT AGC^GCT
AspHI 337 GAGCTC G[AT]GC[AT]^C
BanI 297 GGCGCC G^G[CT][GA]CC
BbeI 297 GGCGCC GGCGC^C
BseRI 262 GAGGAG GAGGAG(10/8)
BsiHKAI 337 GAGCTC G[AT]GC[AT]^C
BsrBI 285 CCGCTC CCGCTC(-3/-3)
Bsu36I 92 CCTCAGG CC^T[ACGT]AGG
DdeI 93 CTCAG C^T[ACGT]AG
DsaI 230 CCGCGG C^C[GA][CT]GG
EaeI 330 TGGCCG [CT]^GGCC[GA]
EarI 396 GAAGAG CTCTTC(1/4)
Eco47III 323 AGCGCT AGC^GCT
Eco81I 92 CCTCAGG CC^T[ACGT]AGG
HphI 147 TCACC GGTGA(8/7)
KasI 297 GGCGCC G^GCGCC
Ksp632I 396 GAAGAG CTCTTC(1/4)
KspI 230 CCGCGG CCGC^GG
MseI 374 TTAA T^TAA
NarI 297 GGCGCC GG^CGCC
NlaIII 181 CATG CATG^
PflMI 305 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 50 CTGCAG CTGCA^G
PvuII 435 CAGCTG CAG^CTG
SacI 337 GAGCTC GAGCT^C
SacII 230 CCGCGG CCGC^GG
SapI 396 GAAGAGC GCTCTTC(1/4)
SfcI 50 CTGCAG C^T[GA][CT]AG
SfoI 297 GGCGCC GGC^GCC
StuI 126 AGGCCT AGG^CCT
Tru9I 374 TTAA T^TAA
Van91I 305 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 124 CCAGGCCTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 206 TCCGGA T^CCGGA
341 TCCGGA
AcyI 139 GACGCC G[GA]^CG[CT]C
297 GGCGCC
ApaI 66 GGGCCC GGGCC^C
88 GGGCCC
BbsI 37 GAAGAC GAAGAC(2/6)
448 GTCTTC
BpmI 42 CTCCAG CTGGAG(16/14)
432 CTCCAG
BpuAI 37 GAAGAC GAAGAC(2/6)
448 GTCTTC
BsaHI 139 GACGCC G[GA]^CG[CT]C
297 GGCGCC
BsaWI 206 TCCGGA [AT]^CCGG[AT]
341 TCCGGA
BseAI 206 TCCGGA T^CCGGA
341 TCCGGA
BspEI 206 TCCGGA T^CCGGA
341 TCCGGA
BsrI 143 CCAGT ACTGG(1/-1)
453 CCAGT
BstF5I 254 GGATG GGATG(2/0)
347 GGATG
BstYI 8 AGATCC [GA]^GATC[CT]
203 AGATCC
FokI 254 GGATG GGATG(9/13)
347 GGATG
HgaI 139 GACGC GACGC(5/10)
441 GACGC
Hin1I 139 GACGCC G[GA]^CG[CT]C
297 GGCGCC
MaeIII 146 GTCAC ^GT[ACGT]AC
353 GTGAC
MflI 8 AGATCC [GA]^GATC[CT]
203 AGATCC
MroI 206 TCCGGA T^CCGGA
341 TCCGGA
MspA1I 230 CCGCGG C[AC]G^C[GT]G
435 CAGCTG
PshAI 139 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
441 GACGCGAGTC
SfaNI 420 GATGC GCATC(5/9)
108 GCATC
Tsp45I 146 GTCAC ^GT[GC]AC
353 GTGAC
XhoII 8 AGATCC [GA]^GATC[CT]
203 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AlwI BanII
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboI MboII MnlI
MspI MvaI MvnI MwoI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
TseI