Restriction map for
pF1KB8233
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# NO CUT ENZYMES ( 193 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I Tsp45I
TspRI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 399 GCGG CCGC(-3/-1)
AclI 362 AACGTT AA^CGTT
AlwI 129 GGATC GGATC(4/5)
AocI 44 CCTCAGG CC^T[ACGT]AGG
AspHI 229 GAGCAC G[AT]GC[AT]^C
BbvCI 196 GCTGAGG CCTCAGC(-5/-2)
BmyI 229 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 229 GAGCAC G[AT]GC[AT]^C
BsiYI 306 CCACAAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 306 CCACAAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 433 GAGAC GTCTC(1/5)
BsmFI 186 GTCCC GGGAC(10/14)
Bsp1286I 229 GAGCAC G[AGT]GC[ACT]^C
BssSI 232 CACGAG CACGAG(-5/-1)
BstYI 129 GGATCT [GA]^GATC[CT]
Bsu36I 44 CCTCAGG CC^T[ACGT]AGG
DpnII 130 GATC ^GATC
Eco81I 44 CCTCAGG CC^T[ACGT]AGG
EcoRI 100 GAATTC G^AATTC
EcoRV 13 GATATC GAT^ATC
HaeIII 49 GGCC GG^CC
HgaI 179 GACGC GACGC(5/10)
HphI 21 GGTGA GGTGA(8/7)
MaeII 363 ACGT A^CGT
MboI 130 GATC ^GATC
MflI 129 GGATCT [GA]^GATC[CT]
MspA1I 211 CAGCTG C[AC]G^C[GT]G
MwoI 340 GCACAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 130 GATC ^GATC
NlaIII 190 CATG CATG^
PflMI 306 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 179 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 362 AACGTT AA^CGTT
PstI 167 CTGCAG CTGCA^G
PvuII 211 CAGCTG CAG^CTG
Sau3AI 130 GATC ^GATC
SfcI 167 CTGCAG C^T[GA][CT]AG
Van91I 306 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 129 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AcsI 100 GAATTC [GA]^AATT[CT]
146 AAATTC
ApoI 100 GAATTC [GA]^AATT[CT]
146 AAATTC
Asp700I 88 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
241 GAAGTGCTTC
BpmI 31 CTGGAG CTGGAG(16/14)
139 CTGGAG
BsaJI 76 CCTCGG C^C[ACGT][ACGT]GG
137 CCCTGG
BsrI 30 ACTGG ACTGG(1/-1)
357 ACTGG
Cac8I 39 GCGTGC GC[ACGT]^[ACGT]GC
213 GCTGGC
EarI 262 GAAGAG CTCTTC(1/4)
292 GAAGAG
Ksp632I 262 GAAGAG CTCTTC(1/4)
292 GAAGAG
XmnI 88 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
241 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BbvI BssKI BstNI DdeI
EcoRII Fnu4HI HinfI ItaI MboII
MnlI MvaI ScrFI TfiI TseI
Tsp509I