Restriction map for
pF1KB8244
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanII
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FauI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeIII MamI MfeI MflI
MluI MroI MslI MunI MvnI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AvaI 314 CCCGAG C^[CT]CG[GA]G
AvaII 595 GGACC G^G[AT]CC
BalI 44 TGGCCA TGG^CCA
BanI 550 GGCGCC G^G[CT][GA]CC
BbeI 550 GGCGCC GGCGC^C
BbsI 889 GAAGAC GAAGAC(2/6)
BclI 269 TGATCA T^GATCA
BcnI 11 CCGGG CC^[GC]GG
BglI 416 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 303 GGGCAC G[AGT]GC[ACT]^C
BpuAI 889 GAAGAC GAAGAC(2/6)
BsmFI 840 GGGAC GGGAC(10/14)
BsoBI 314 CCCGAG C^[CT]CG[GA]G
Bsp1286I 303 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 645 TGTACA T^GTACA
BspDI 73 ATCGAT AT^CGAT
BsrBI 15 GAGCGG CCGCTC(-3/-3)
BsrGI 645 TGTACA T^GTACA
BstF5I 255 GGATG GGATG(2/0)
CfoI 551 GCGC GCG^C
ClaI 73 ATCGAT AT^CGAT
DraII 594 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 945 CCATGG C^C[GA][CT]GG
EcoNI 586 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 594 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 945 CCATGG C^C[AT][AT]GG
EcoT22I 199 ATGCAT ATGCA^T
FbaI 269 TGATCA T^GATCA
FokI 255 GGATG GGATG(9/13)
HaeII 550 GGCGCC [GA]GCGC^[CT]
HapII 11 CCGG C^CGG
HgaI 7 GCGTC GACGC(5/10)
HhaI 551 GCGC GCG^C
HinP1I 551 GCGC G^CGC
HinfI 543 GAATC G^A[ACGT]TC
HpaII 11 CCGG C^CGG
KasI 550 GGCGCC G^GCGCC
MaeII 745 ACGT A^CGT
MluNI 44 TGGCCA TGG^CCA
MscI 44 TGGCCA TGG^CCA
MspI 11 CCGG C^CGG
NarI 550 GGCGCC GG^CGCC
NciI 11 CCGGG CC^[GC]GG
NcoI 945 CCATGG C^CATGG
NdeI 338 CATATG CA^TATG
NsiI 199 ATGCAT ATGCA^T
PpuMI 594 AGGACCT [GA]G^G[AT]CC[CT]
SfoI 550 GGCGCC GGC^GCC
SmlI 712 CTTGAG C^T[CT][GA]AG
SphI 201 GCATGC GCATG^C
SspBI 645 TGTACA T^GTACA
SspI 659 AATATT AAT^ATT
StyI 945 CCATGG C^C[AT][AT]GG
TaqI 74 TCGA T^CGA
TfiI 543 GAATC G^A[AT]TC
TthHB8I 74 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcyI 6 GGCGTC G[GA]^CG[CT]C
550 GGCGCC
BfaI 244 CTAG C^TAG
758 CTAG
BsaHI 6 GGCGTC G[GA]^CG[CT]C
550 GGCGCC
Cfr13I 24 GGCCC G^G[ACGT]CC
595 GGACC
EaeI 44 TGGCCA [CT]^GGCC[GA]
381 TGGCCG
Hin1I 6 GGCGTC G[GA]^CG[CT]C
550 GGCGCC
MaeI 244 CTAG C^TAG
758 CTAG
MspA1I 835 CAGCTG C[AC]G^C[GT]G
918 CAGCTG
NlaIV 486 GGAACC GG[ACGT]^[ACGT]CC
550 GGCGCC
NspI 53 ACATGC [GA]CATG^[CT]
201 GCATGC
PvuII 835 CAGCTG CAG^CTG
918 CAGCTG
Sau96I 24 GGCCC G^G[ACGT]CC
595 GGACC
ScaI 158 AGTACT AGT^ACT
457 AGTACT
Tsp509I 707 AATT ^AATT
990 AATT
XcmI 266 CCATGATCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
417 CCACCCTGGCTATGG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI HaeIII HphI ItaI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
ScrFI SfaNI Tru9I TseI TspRI