##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        TfiI        Tsp45I      Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI           38  GCGG                 CCGC(-3/-1)
AflII         589  CTTAAG               C^TTAAG
BbvI          553  GCTGC                GCAGC(8/12)
BfrI          589  CTTAAG               C^TTAAG
BmyI          124  GGGCAC               G[AGT]GC[ACT]^C
Bsp1286I      124  GGGCAC               G[AGT]GC[ACT]^C
BsrI          198  CCAGT                ACTGG(1/-1)
BstBI         286  TTCGAA               TT^CGAA
BstF5I        274  GGATG                GGATG(2/0)
Cfr13I         25  GGGCC                G^G[ACGT]CC
DdeI          557  CTAAG                C^T[ACGT]AG
EaeI           14  TGGCCG               [CT]^GGCC[GA]
EcoRI         283  GAATTC               G^AATTC
FauI           38  GCGGG                CCCGC(4/6)
Fnu4HI        553  GCTGC                GC^[ACGT]GC
FokI          274  GGATG                GGATG(9/13)
HapII          35  CCGG                 C^CGG
HindIII       570  AAGCTT               A^AGCTT
HinfI          31  GACTC                G^A[ACGT]TC
HpaII          35  CCGG                 C^CGG
HphI          280  GGTGA                GGTGA(8/7)
ItaI          553  GCTGC                GC^[ACGT]GC
MspI           35  CCGG                 C^CGG
NlaIV         490  GGAGCC               GG[ACGT]^[ACGT]CC
NspV          286  TTCGAA               TT^CGAA
PleI           31  GACTC                GAGTC(4/5)
Sau96I         25  GGGCC                G^G[ACGT]CC
SfaNI         450  GATGC                GCATC(5/9)
SfuI          286  TTCGAA               TT^CGAA
SmlI          589  CTTAAG               C^T[CT][GA]AG
SspI          303  AATATT               AAT^ATT
TaqI          287  TCGA                 T^CGA
TseI          553  GCTGC                G^C[AT]GC
TthHB8I       287  TCGA                 T^CGA
XcmI          374  CCAATGCAATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BsiYI          17  CCGAATTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               28  CCTGACTCCGG         
BslI           17  CCGAATTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               28  CCTGACTCCGG         
BsrDI         379  GCAATG               GCAATG(2/0)
              445  GCAATG              
DraI          202  TTTAAA               TTT^AAA
              630  TTTAAA              
HaeIII         15  GGCC                 GG^CC
               26  GGCC                
MnlI          250  CCTC                 CCTC(7/6)
              169  GAGG                
NdeI          182  CATATG               CA^TATG
              254  CATATG              
NlaIII        211  CATG                 CATG^
              502  CATG                
TspRI         136  CAGTG                CA[GC]TG[ACGT][ACGT]^
              398  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AcsI        AluI        ApoI        MaeIII      MboII       
MseI        Tru9I       Tsp509I