Restriction map for
pF1KB8252
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
Ksp632I MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccII 598 CGCG CG^CG
AfaI 279 GTAC GT^AC
AhdI 735 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 241 GATCC GGATC(4/5)
ApaI 801 GGGCCC GGGCC^C
AspEI 735 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 832 GAGCAC G[AT]GC[AT]^C
BalI 479 TGGCCA TGG^CCA
BbeI 44 GGCGCC GGCGC^C
BcnI 575 CCGGG CC^[GC]GG
BfaI 745 CTAG C^TAG
BglI 985 GCCCACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 467 GCTTAGC GC^T[ACGT]AGC
BpmI 607 CTCCAG CTGGAG(16/14)
Bpu1102I 467 GCTTAGC GC^T[ACGT]AGC
BsaI 843 GGTCTC GGTCTC(1/5)
BsgI 453 CTGCAC GTGCAG(16/14)
BsiHKAI 832 GAGCAC G[AT]GC[AT]^C
BsmAI 844 GTCTC GTCTC(1/5)
BsmFI 703 GTCCC GGGAC(10/14)
BspHI 521 TCATGA T^CATGA
BspLU11I 134 ACATGT A^CATGT
BstEII 51 GGTCACC G^GT[ACGT]ACC
BstPI 51 GGTCACC G^GT[ACGT]ACC
BstUI 598 CGCG CG^CG
BstXI 641 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 240 AGATCC [GA]^GATC[CT]
CelII 467 GCTTAGC GC^T[ACGT]AGC
DraIII 835 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 913 GACCACTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 735 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 477 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 51 GGTCACC G^GT[ACGT]ACC
FauI 599 GCGGG CCCGC(4/6)
HapII 575 CCGG C^CGG
HincII 22 GTCAAC GT[CT]^[GA]AC
HindII 22 GTCAAC GT[CT]^[GA]AC
HindIII 196 AAGCTT A^AGCTT
HpaII 575 CCGG C^CGG
KasI 44 GGCGCC G^GCGCC
KspI 597 CCGCGG CCGC^GG
MaeI 745 CTAG C^TAG
MaeIII 52 GTCAC ^GT[ACGT]AC
MflI 240 AGATCC [GA]^GATC[CT]
MluNI 479 TGGCCA TGG^CCA
MscI 479 TGGCCA TGG^CCA
MseI 68 TTAA T^TAA
MspA1I 597 CCGCGG C[AC]G^C[GT]G
MspI 575 CCGG C^CGG
MvnI 598 CGCG CG^CG
NarI 44 GGCGCC GG^CGCC
NciI 575 CCGGG CC^[GC]GG
NspI 134 ACATGT [GA]CATG^[CT]
PshAI 525 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 521 TCATGA T^CATGA
RsaI 279 GTAC GT^AC
SacII 597 CCGCGG CCGC^GG
SexAI 836 ACCAGGT A^CC[AT]GGT
SfcI 957 CTACAG C^T[GA][CT]AG
SfiI 984 GGCCCACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 44 GGCGCC GGC^GCC
TfiI 148 GATTC G^A[AT]TC
Tru9I 68 TTAA T^TAA
Tsp45I 52 GTCAC ^GT[GC]AC
XbaI 744 TCTAGA T^CTAGA
XhoII 240 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 597 CCGC CCGC(-3/-1)
599 GCGG
AcsI 163 AAATTC [GA]^AATT[CT]
949 GAATTT
AflIII 134 ACATGT A^C[GA][CT]GT
716 ACGTGT
ApoI 163 AAATTC [GA]^AATT[CT]
949 GAATTT
AvaII 729 GGACC G^G[AT]CC
975 GGACC
BanI 44 GGCGCC G^G[CT][GA]CC
172 GGCACC
BbsI 555 GAAGAC GAAGAC(2/6)
301 GTCTTC
BbvI 61 GCTGC GCAGC(8/12)
637 GCTGC
BpuAI 555 GAAGAC GAAGAC(2/6)
301 GTCTTC
BseRI 846 CTCCTC GAGGAG(10/8)
873 CTCCTC
BspMI 736 ACCTGC ACCTGC(4/8)
753 ACCTGC
BsrDI 900 CATTGC GCAATG(2/0)
443 GCAATG
DpnII 241 GATC ^GATC
435 GATC
DraII 800 AGGGCCC [GA]G^G[ACGT]CC[CT]
801 GGGCCCC
EaeI 479 TGGCCA [CT]^GGCC[GA]
594 TGGCCG
EcoO109I 800 AGGGCCC [GA]G^G[ACGT]CC[CT]
801 GGGCCCC
MaeII 281 ACGT A^CGT
716 ACGT
MboI 241 GATC ^GATC
435 GATC
NdeII 241 GATC ^GATC
435 GATC
Sau3AI 241 GATC ^GATC
435 GATC
SmlI 430 CTCAAG C^T[CT][GA]AG
808 CTCAAG
TseI 61 GCTGC G^C[AT]GC
637 GCTGC
Tsp509I 164 AATT ^AATT
950 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcyI AluI BanII BmyI BsaHI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DsaI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HinfI HphI
ItaI MboII MnlI MslI MvaI
MwoI NcoI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI StyI TspRI
XcmI