Restriction map for
pF1KB8254
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiWI BsiYI
BslI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TseI Tsp45I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AgeI 147 ACCGGT A^CCGGT
AlwI 516 GATCC GGATC(4/5)
AocI 295 CCTGAGG CC^T[ACGT]AGG
AspHI 468 GTGCTC G[AT]GC[AT]^C
BanI 305 GGCACC G^G[CT][GA]CC
BclI 59 TGATCA T^GATCA
BmyI 468 GTGCTC G[AGT]GC[ACT]^C
BsaJI 431 CCATGG C^C[ACGT][ACGT]GG
BsaWI 147 ACCGGT [AT]^CCGG[AT]
BsiEI 374 CGATCG CG[GA][CT]^CG
BsiHKAI 468 GTGCTC G[AT]GC[AT]^C
BsmAI 29 GAGAC GTCTC(1/5)
BsmBI 29 GAGACG CGTCTC(1/5)
BsmFI 314 GTCCC GGGAC(10/14)
Bsp1286I 468 GTGCTC G[AGT]GC[ACT]^C
BsrFI 147 ACCGGT [GA]^CCGG[CT]
BstXI 125 CCAATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
CfoI 475 GCGC GCG^C
Cfr10I 147 ACCGGT [GA]^CCGG[CT]
DsaI 431 CCATGG C^C[GA][CT]GG
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
EcoT14I 431 CCATGG C^C[AT][AT]GG
EcoT22I 77 ATGCAT ATGCA^T
FauI 534 CCCGC CCCGC(4/6)
FbaI 59 TGATCA T^GATCA
Fnu4HI 303 GCGGC GC^[ACGT]GC
HaeII 474 GGCGCT [GA]GCGC^[CT]
HapII 148 CCGG C^CGG
HhaI 475 GCGC GCG^C
HinP1I 475 GCGC G^CGC
HpaII 148 CCGG C^CGG
ItaI 303 GCGGC GC^[ACGT]GC
MaeIII 65 GTTAC ^GT[ACGT]AC
MseI 235 TTAA T^TAA
MslI 330 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 173 CAGCTG C[AC]G^C[GT]G
MspI 148 CCGG C^CGG
NcoI 431 CCATGG C^CATGG
NlaIII 432 CATG CATG^
NlaIV 305 GGCACC GG[ACGT]^[ACGT]CC
NsiI 77 ATGCAT ATGCA^T
PinAI 147 ACCGGT A^CCGGT
PvuI 374 CGATCG CGAT^CG
PvuII 173 CAGCTG CAG^CTG
SfaNI 262 GCATC GCATC(5/9)
SfcI 38 CTGTAG C^T[GA][CT]AG
SmlI 493 CTCAAG C^T[CT][GA]AG
StuI 489 AGGCCT AGG^CCT
StyI 431 CCATGG C^C[AT][AT]GG
TaqI 377 TCGA T^CGA
TfiI 153 GAATC G^A[AT]TC
Tru9I 235 TTAA T^TAA
TthHB8I 377 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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BciVI 168 GGATAC GTATCC(6/5)
291 GTATCC
BfaI 334 CTAG C^TAG
427 CTAG
BpmI 86 CTGGAG CTGGAG(16/14)
463 CTGGAG
BssKI 85 CCTGG ^CC[ACGT]GG
462 CCTGG
BstF5I 330 CATCC GGATG(2/0)
360 GGATG
BstNI 85 CCTGG CC^[AT]GG
462 CCTGG
EarI 549 GAAGAG CTCTTC(1/4)
555 GAAGAG
EcoRII 85 CCTGG ^CC[AT]GG
462 CCTGG
FokI 330 CATCC GGATG(9/13)
360 GGATG
HaeIII 6 GGCC GG^CC
490 GGCC
HphI 150 GGTGA GGTGA(8/7)
410 TCACC
Ksp632I 549 GAAGAG CTCTTC(1/4)
555 GAAGAG
MaeI 334 CTAG C^TAG
427 CTAG
MvaI 85 CCTGG CC^[AT]GG
462 CCTGG
MwoI 477 GCTCACACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
514 GCGATCCGAGC
PleI 402 GACTC GAGTC(4/5)
255 GAGTC
ScrFI 85 CCTGG CC^[ACGT]GG
462 CCTGG
Tsp509I 115 AATT ^AATT
237 AATT
TspRI 310 CACTG CA[GC]TG[ACGT][ACGT]^
500 CAGTG
XcmI 8 CCATCAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
326 CCATCATCCTAGTGG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AciI AluI Cac8I DdeI DpnII
HinfI MboI MboII MnlI NdeII
Sau3AI