Restriction map for
pF1KB8265
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AgeI 388 ACCGGT A^CCGGT
Alw44I 384 GTGCAC G^TGCAC
ApaLI 384 GTGCAC G^TGCAC
Asp700I 431 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 181 CACGTG CAC^GTG
BbsI 392 GTCTTC GAAGAC(2/6)
BbvCI 1153 GCTGAGG CCTCAGC(-5/-2)
BglI 1040 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1131 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1131 GCTGAGC GC^T[ACGT]AGC
BpuAI 392 GTCTTC GAAGAC(2/6)
BsaI 1097 GAGACC GGTCTC(1/5)
BsaWI 388 ACCGGT [AT]^CCGG[AT]
BsrFI 388 ACCGGT [GA]^CCGG[CT]
BstAPI 50 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1131 GCTGAGC GC^T[ACGT]AGC
Cfr10I 388 ACCGGT [GA]^CCGG[CT]
EarI 256 CTCTTC CTCTTC(1/4)
EcoRV 652 GATATC GAT^ATC
FauI 788 CCCGC CCCGC(4/6)
HincII 748 GTTGAC GT[CT]^[GA]AC
HindII 748 GTTGAC GT[CT]^[GA]AC
Ksp632I 256 CTCTTC CTCTTC(1/4)
MseI 680 TTAA T^TAA
MslI 1080 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 536 CAGCTG C[AC]G^C[GT]G
NcoI 924 CCATGG C^CATGG
NdeI 812 CATATG CA^TATG
NspI 1127 GCATGC [GA]CATG^[CT]
PflMI 1023 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 388 ACCGGT A^CCGGT
PleI 139 GACTC GAGTC(4/5)
PmaCI 181 CACGTG CAC^GTG
PmlI 181 CACGTG CAC^GTG
PshAI 631 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 536 CAGCTG CAG^CTG
SacI 64 GAGCTC GAGCT^C
SapI 255 GCTCTTC GCTCTTC(1/4)
SexAI 773 ACCTGGT A^CC[AT]GGT
SmaI 188 CCCGGG CCC^GGG
SmlI 1282 CTTGAG C^T[CT][GA]AG
SphI 1127 GCATGC GCATG^C
StuI 1157 AGGCCT AGG^CCT
TfiI 1312 GATTC G^A[AT]TC
Tru9I 680 TTAA T^TAA
Van91I 1023 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 414 CCACAGCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
XmnI 431 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 202 GTAGAC GT^[AC][GT]AC
610 GTAGAC
AcsI 362 GAATTC [GA]^AATT[CT]
760 GAATTC
ApoI 362 GAATTC [GA]^AATT[CT]
760 GAATTC
AvaI 188 CCCGGG C^[CT]CG[GA]G
311 CCCGAG
AvaII 357 GGACC G^G[AT]CC
1173 GGACC
BpmI 499 CTGGAG CTGGAG(16/14)
583 CTGGAG
BsaAI 181 CACGTG [CT]AC^GT[GA]
1222 TACGTG
BseRI 131 GAGGAG GAGGAG(10/8)
1297 GAGGAG
BsmBI 466 CGTCTC CGTCTC(1/5)
163 GAGACG
BsoBI 188 CCCGGG C^[CT]CG[GA]G
311 CCCGAG
DpnII 339 GATC ^GATC
870 GATC
DsaI 924 CCATGG C^C[GA][CT]GG
957 CCGTGG
EcoNI 1101 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1281 CCTTGAGAAGG
EcoRI 362 GAATTC G^AATTC
760 GAATTC
EcoT14I 924 CCATGG C^C[AT][AT]GG
1106 CCAAGG
HaeII 169 GGCGCT [GA]GCGC^[CT]
659 AGCGCC
HinfI 139 GACTC G^A[ACGT]TC
1312 GATTC
MboI 339 GATC ^GATC
870 GATC
NdeII 339 GATC ^GATC
870 GATC
Sau3AI 339 GATC ^GATC
870 GATC
SfcI 539 CTGTAG C^T[GA][CT]AG
860 CTGTAG
StyI 924 CCATGG C^C[AT][AT]GG
1106 CCAAGG
TaqI 650 TCGA T^CGA
656 TCGA
Tsp509I 363 AATT ^AATT
761 AATT
TthHB8I 650 TCGA T^CGA
656 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AspHI BalI
BanI BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboII
MluNI MnlI MscI MspI MvaI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI