##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SspBI       SspI        StyI        
SwaI        Tru9I       TspRI       Tth111I     XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AcsI          114  GAATTT               [GA]^AATT[CT]
ApoI          114  GAATTT               [GA]^AATT[CT]
AspHI         545  GAGCAC               G[AT]GC[AT]^C
AvaI          278  CTCGAG               C^[CT]CG[GA]G
BamHI         611  GGATCC               G^GATCC
BanI           85  GGCACC               G^G[CT][GA]CC
BsiEI          69  CGGCCG               CG[GA][CT]^CG
BsiHKAI       545  GAGCAC               G[AT]GC[AT]^C
BsmI          656  GAATGC               GAATGC(1/-1)
BsoBI         278  CTCGAG               C^[CT]CG[GA]G
EagI           69  CGGCCG               C^GGCCG
EclXI          69  CGGCCG               C^GGCCG
Eco52I         69  CGGCCG               C^GGCCG
EcoNI         619  CCTGGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          404  GCGGG                CCCGC(4/6)
HphI          420  GGTGA                GGTGA(8/7)
MaeIII        128  GTGAC                ^GT[ACGT]AC
NspI          641  GCATGC               [GA]CATG^[CT]
PaeR7I        278  CTCGAG               C^TCGAG
SmlI          278  CTCGAG               C^T[CT][GA]AG
SphI          641  GCATGC               GCATG^C
StuI          684  AGGCCT               AGG^CCT
TaqI          279  TCGA                 T^CGA
Tsp45I        128  GTGAC                ^GT[GC]AC
Tsp509I       115  AATT                 ^AATT
TthHB8I       279  TCGA                 T^CGA
XcmI          498  CCAGTACCCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          278  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          443  GTAC                 GT^AC
              501  GTAC                
AlwNI         452  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              461  CAGCTGCTG           
ApaI          306  GGGCCC               GGGCC^C
              567  GGGCCC              
BpmI          554  CTCCAG               CTGGAG(16/14)
              293  CTGGAG              
BsaI          585  GAGACC               GGTCTC(1/5)
              231  GGTCTC              
BsmAI         585  GAGAC                GTCTC(1/5)
              232  GTCTC               
BstYI          74  GGATCT               [GA]^GATC[CT]
              611  GGATCC              
DsaI          393  CCGTGG               C^C[GA][CT]GG
              513  CCGTGG              
EaeI           69  CGGCCG               [CT]^GGCC[GA]
              510  TGGCCG              
MflI           74  GGATCT               [GA]^GATC[CT]
              611  GGATCC              
PleI          630  GACTC                GAGTC(4/5)
              194  GAGTC               
PvuII         344  CAGCTG               CAG^CTG
              461  CAGCTG              
RsaI          443  GTAC                 GT^AC
              501  GTAC                
SbfI           60  CCTGCAGG             CCTGCA^GG
              370  CCTGCAGG            
Sse8387I       60  CCTGCAGG             CCTGCA^GG
              370  CCTGCAGG            
TfiI          225  GAATC                G^A[AT]TC
              338  GAATC               
XhoII          74  GGATCT               [GA]^GATC[CT]
              611  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
Cfr13I      DpnII       DraII       EarI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        Ksp632I     MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PpuMI       PstI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TseI        Van91I