##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BbeI        BbrPI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MvaI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII         231  CGCG                 CG^CG
AluI          243  AGCT                 AG^CT
ApaI          226  GGGCCC               GGGCC^C
AvaII          45  GGACC                G^G[AT]CC
BamHI         267  GGATCC               G^GATCC
BanII         226  GGGCCC               G[GA]GC[CT]^C
BbsI          282  GAAGAC               GAAGAC(2/6)
BbvCI         133  GCTGAGG              CCTCAGC(-5/-2)
BmyI          226  GGGCCC               G[AGT]GC[ACT]^C
BpuAI         282  GAAGAC               GAAGAC(2/6)
BsiEI          26  CGGCCG               CG[GA][CT]^CG
Bsp1286I      226  GGGCCC               G[AGT]GC[ACT]^C
BstUI         231  CGCG                 CG^CG
BstYI         267  GGATCC               [GA]^GATC[CT]
DdeI          134  CTGAG                C^T[ACGT]AG
DpnII         268  GATC                 ^GATC
EaeI           26  CGGCCG               [CT]^GGCC[GA]
EagI           26  CGGCCG               C^GGCCG
EclXI          26  CGGCCG               C^GGCCG
Eco52I         26  CGGCCG               C^GGCCG
EcoT14I        41  CCAAGG               C^C[AT][AT]GG
FauI          229  CCCGC                CCCGC(4/6)
HaeII          37  AGCGCC               [GA]GCGC^[CT]
HapII          87  CCGG                 C^CGG
HpaII          87  CCGG                 C^CGG
MboI          268  GATC                 ^GATC
MflI          267  GGATCC               [GA]^GATC[CT]
MspA1I         23  CAGCGG               C[AC]G^C[GT]G
MspI           87  CCGG                 C^CGG
MvnI          231  CGCG                 CG^CG
NdeII         268  GATC                 ^GATC
PpuMI          44  AGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI        268  GATC                 ^GATC
SfaNI         298  GCATC                GCATC(5/9)
StyI           41  CCAAGG               C^C[AT][AT]GG
XhoII         267  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AlwI          268  GATCC                GGATC(4/5)
              267  GGATC               
BbvI           22  GCAGC                GCAGC(8/12)
              244  GCTGC               
BsaJI          41  CCAAGG               C^C[ACGT][ACGT]GG
              158  CCGAGG              
Cac8I          82  GCAAGC               GC[ACGT]^[ACGT]GC
              228  GCCCGC              
CfoI           38  GCGC                 GCG^C
              232  GCGC                
DraII          44  AGGACCT              [GA]G^G[ACGT]CC[CT]
              225  AGGGCCC             
EcoO109I       44  AGGACCT              [GA]G^G[ACGT]CC[CT]
              225  AGGGCCC             
HaeIII         27  GGCC                 GG^CC
              227  GGCC                
HhaI           38  GCGC                 GCG^C
              232  GCGC                
HinP1I         38  GCGC                 G^CGC
              232  GCGC                
NlaIV         226  GGGCCC               GG[ACGT]^[ACGT]CC
              267  GGATCC              
TseI           22  GCAGC                G^C[AT]GC
              244  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AciI        BseRI       BstF5I      Cfr13I      EarI        
Fnu4HI      FokI        ItaI        Ksp632I     MboII       
MnlI        Sau96I