Restriction map for
pF1KB8284
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AvrII BbeI BbrPI
BbvCI BcgI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsgI BsiEI BsiWI BsmI BspDI
BspEI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI HaeII Hin1I
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI Tth111I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 556 GGTACC G^GTACC
AccI 1171 GTCGAC GT^[AC][GT]AC
AccII 18 CGCG CG^CG
AhdI 493 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 936 GGGCCC GGGCC^C
Asp718I 556 GGTACC G^GTACC
AspEI 493 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 61 GAGCAC G[AT]GC[AT]^C
AviII 761 TGCGCA TGC^GCA
BalI 470 TGGCCA TGG^CCA
BamHI 8 GGATCC G^GATCC
BanI 556 GGTACC G^G[CT][GA]CC
BanII 936 GGGCCC G[GA]GC[CT]^C
BbsI 501 GTCTTC GAAGAC(2/6)
BciVI 1029 GTATCC GTATCC(6/5)
BglI 165 GCCGCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1109 AGATCT A^GATCT
BpuAI 501 GTCTTC GAAGAC(2/6)
BseRI 737 GAGGAG GAGGAG(10/8)
BsiHKAI 61 GAGCAC G[AT]GC[AT]^C
BsmBI 189 GAGACG CGTCTC(1/5)
Bsp1407I 107 TGTACA T^GTACA
BspHI 1094 TCATGA T^CATGA
BsrDI 354 CATTGC GCAATG(2/0)
BsrFI 170 ACCGGC [GA]^CCGG[CT]
BsrGI 107 TGTACA T^GTACA
BssHII 17 GCGCGC G^CGCGC
BssSI 478 CACGAG CACGAG(-5/-1)
BstUI 18 CGCG CG^CG
BstXI 587 CCAAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 170 ACCGGC [GA]^CCGG[CT]
DraI 1215 TTTAAA TTT^AAA
DraIII 526 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1174 GACTCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 792 CCACGG C^C[GA][CT]GG
Eam1105I 493 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1176 CTCTTC CTCTTC(1/4)
FauI 31 CCCGC CCCGC(4/6)
FspI 761 TGCGCA TGC^GCA
HgaI 797 GACGC GACGC(5/10)
HincII 1171 GTCGAC GT[CT]^[GA]AC
HindII 1171 GTCGAC GT[CT]^[GA]AC
KpnI 556 GGTACC GGTAC^C
Ksp632I 1176 CTCTTC CTCTTC(1/4)
MaeII 192 ACGT A^CGT
MluNI 470 TGGCCA TGG^CCA
MscI 470 TGGCCA TGG^CCA
MvnI 18 CGCG CG^CG
PaeR7I 687 CTCGAG C^TCGAG
PflMI 525 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 220 CAGCTG CAG^CTG
RcaI 1094 TCATGA T^CATGA
SalI 1171 GTCGAC G^TCGAC
SmaI 449 CCCGGG CCC^GGG
SmlI 687 CTCGAG C^T[CT][GA]AG
SspBI 107 TGTACA T^GTACA
StuI 1059 AGGCCT AGG^CCT
TfiI 742 GAATC G^A[AT]TC
Van91I 525 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 687 CTCGAG C^TCGAG
XmaCI 449 CCCGGG C^CCGGG
XmaI 449 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 95 ACATGT A^C[GA][CT]GT
442 ACATGT
AlwNI 463 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
992 CAGCGACTG
AvaII 492 GGACC G^G[AT]CC
640 GGTCC
BclI 333 TGATCA T^GATCA
917 TGATCA
BfaI 905 CTAG C^TAG
1088 CTAG
BsaWI 197 ACCGGA [AT]^CCGG[AT]
869 ACCGGA
BsmFI 54 GGGAC GGGAC(10/14)
491 GGGAC
BspLU11I 95 ACATGT A^CATGT
442 ACATGT
BspMI 1004 ACCTGC ACCTGC(4/8)
800 GCAGGT
BsrBI 4 GAGCGG CCGCTC(-3/-3)
702 GAGCGG
DraII 491 GGGACCT [GA]G^G[ACGT]CC[CT]
639 AGGTCCT
EaeI 470 TGGCCA [CT]^GGCC[GA]
623 CGGCCA
EcoO109I 491 GGGACCT [GA]G^G[ACGT]CC[CT]
639 AGGTCCT
FbaI 333 TGATCA T^GATCA
917 TGATCA
HphI 382 GGTGA GGTGA(8/7)
515 TCACC
MaeI 905 CTAG C^TAG
1088 CTAG
MslI 685 CACTCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1035 CACCTCAGTG
MspA1I 220 CAGCTG C[AC]G^C[GT]G
670 CAGCGG
PpuMI 491 GGGACCT [GA]G^G[AT]CC[CT]
639 AGGTCCT
PstI 361 CTGCAG CTGCA^G
547 CTGCAG
SfaNI 873 GATGC GCATC(5/9)
212 GCATC
SfcI 361 CTGCAG C^T[GA][CT]AG
547 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BbvI BcnI BmyI BpmI BsaI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII