################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AfaI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 512 GTAGAC GT^[AC][GT]AC AclI 671 AACGTT AA^CGTT AflII 709 CTTAAG C^TTAAG AlwNI 749 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 115 GAGCAC G[AT]GC[AT]^C AvaII 445 GGACC G^G[AT]CC BamHI 82 GGATCC G^GATCC BanII 165 GAGCCC G[GA]GC[CT]^C BfrI 709 CTTAAG C^TTAAG BpmI 772 CTGGAG CTGGAG(16/14) BsiHKAI 115 GAGCAC G[AT]GC[AT]^C BsrDI 237 CATTGC GCAATG(2/0) BsrI 730 ACTGG ACTGG(1/-1) BstXI 764 CCAATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 445 GGACC G^G[ACGT]CC DraIII 335 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 295 GAAGAG CTCTTC(1/4) FauI 49 GCGGG CCCGC(4/6) HaeIII 185 GGCC GG^CC HgaI 18 GACGC GACGC(5/10) HincII 208 GTTGAC GT[CT]^[GA]AC HindII 208 GTTGAC GT[CT]^[GA]AC HindIII 320 AAGCTT A^AGCTT HphI 112 GGTGA GGTGA(8/7) Ksp632I 295 GAAGAG CTCTTC(1/4) MaeII 672 ACGT A^CGT MaeIII 486 GTCAC ^GT[ACGT]AC NlaIII 101 CATG CATG^ NlaIV 82 GGATCC GG[ACGT]^[ACGT]CC NspI 100 ACATGC [GA]CATG^[CT] Psp1406I 671 AACGTT AA^CGTT PstI 58 CTGCAG CTGCA^G Sau96I 445 GGACC G^G[ACGT]CC SfaNI 526 GATGC GCATC(5/9) SpeI 589 ACTAGT A^CTAGT StuI 184 AGGCCT AGG^CCT Tsp45I 486 GTCAC ^GT[GC]AC TspRI 22 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 621 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 269 AAATTC [GA]^AATT[CT] 819 AAATTC AluI 321 AGCT AG^CT 371 AGCT AlwI 83 GATCC GGATC(4/5) 82 GGATC ApoI 269 AAATTC [GA]^AATT[CT] 819 AAATTC Asp700I 496 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 688 GAAGACATTC BbsI 688 GAAGAC GAAGAC(2/6) 726 GAAGAC BglII 255 AGATCT A^GATCT 618 AGATCT BmyI 115 GAGCAC G[AGT]GC[ACT]^C 165 GAGCCC BpuAI 688 GAAGAC GAAGAC(2/6) 726 GAAGAC BsaJI 168 CCCAGG C^C[ACGT][ACGT]GG 350 CCAGGG BsgI 332 CTGCAC GTGCAG(16/14) 400 GTGCAG BsmI 653 GAATGC GAATGC(1/-1) 879 GCATTC Bsp1286I 115 GAGCAC G[AGT]GC[ACT]^C 165 GAGCCC BssKI 169 CCAGG ^CC[ACGT]GG 350 CCAGG BstBI 460 TTCGAA TT^CGAA 503 TTCGAA BstNI 169 CCAGG CC^[AT]GG 350 CCAGG EcoRII 169 CCAGG ^CC[AT]GG 350 CCAGG MvaI 169 CCAGG CC^[AT]GG 350 CCAGG NspV 460 TTCGAA TT^CGAA 503 TTCGAA PleI 515 GACTC GAGTC(4/5) 900 GACTC ScrFI 169 CCAGG CC^[ACGT]GG 350 CCAGG SfcI 58 CTGCAG C^T[GA][CT]AG 530 CTACAG SfuI 460 TTCGAA TT^CGAA 503 TTCGAA SmlI 105 CTCAAG C^T[CT][GA]AG 709 CTTAAG XmnI 496 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 688 GAAGACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI BbvI BfaI BstF5I BstYI DdeI DpnII Fnu4HI FokI HinfI ItaI MaeI MboI MboII MflI MnlI MseI MwoI NdeII Sau3AI TaqI TfiI Tru9I TseI Tsp509I TthHB8I XhoII