################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AflII ApoI AscI AseI AsnI AspI AviII BalI BbrPI BbsI BciVI BclI BfrI BglI BlpI Bpu1102I BpuAI BsaBI BseAI BsiEI BsiWI BsmFI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DrdI DsaI EaeI EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfaNI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 155 GGTACC G^GTACC AcyI 1071 GGCGCC G[GA]^CG[CT]C AflIII 914 ACGTGT A^C[GA][CT]GT AgeI 426 ACCGGT A^CCGGT AhdI 392 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 918 GTGCAC G^TGCAC ApaLI 918 GTGCAC G^TGCAC Asp718I 155 GGTACC G^GTACC AspEI 392 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 40 CCTAGG C^CTAGG BamHI 1139 GGATCC G^GATCC BbeI 1071 GGCGCC GGCGC^C BcgI 473 CGAGCAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 363 AGATCT A^GATCT BlnI 40 CCTAGG C^CTAGG BsaAI 913 TACGTG [CT]AC^GT[GA] BsaHI 1071 GGCGCC G[GA]^CG[CT]C BsgI 313 GTGCAG GTGCAG(16/14) BsmBI 822 GAGACG CGTCTC(1/5) BsmI 1055 GAATGC GAATGC(1/-1) Bsp1407I 533 TGTACA T^GTACA BspMI 57 GCAGGT ACCTGC(4/8) BsrBI 370 GAGCGG CCGCTC(-3/-3) BsrDI 322 CATTGC GCAATG(2/0) BsrGI 533 TGTACA T^GTACA BssSI 613 CACGAG CACGAG(-5/-1) BstXI 540 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 392 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 441 GATATC GAT^ATC Hin1I 1071 GGCGCC G[GA]^CG[CT]C KasI 1071 GGCGCC G^GCGCC KpnI 155 GGTACC GGTAC^C MseI 1043 TTAA T^TAA MspA1I 1107 CAGCGG C[AC]G^C[GT]G NarI 1071 GGCGCC GG^CGCC PinAI 426 ACCGGT A^CCGGT SapI 1182 GAAGAGC GCTCTTC(1/4) SexAI 395 ACCAGGT A^CC[AT]GGT SfoI 1071 GGCGCC GGC^GCC SmlI 569 CTCAAG C^T[CT][GA]AG SspBI 533 TGTACA T^GTACA Tru9I 1043 TTAA T^TAA Tsp509I 672 AATT ^AATT XcmI 159 CCATGATACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AocI 449 CCTCAGG CC^T[ACGT]AGG 697 CCTGAGG Aor51HI 137 AGCGCT AGC^GCT 334 AGCGCT ApaI 36 GGGCCC GGGCC^C 526 GGGCCC BanI 155 GGTACC G^G[CT][GA]CC 1071 GGCGCC BbvCI 1206 CCTCAGC CCTCAGC(-5/-2) 219 GCTGAGG BsaI 499 GAGACC GGTCTC(1/5) 399 GGTCTC BsaWI 426 ACCGGT [AT]^CCGG[AT] 1231 ACCGGA BsrFI 426 ACCGGT [GA]^CCGG[CT] 989 ACCGGC Bsu36I 449 CCTCAGG CC^T[ACGT]AGG 697 CCTGAGG Cfr10I 426 ACCGGT [GA]^CCGG[CT] 989 ACCGGC DraIII 921 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 979 CACTTGGTG Eco47III 137 AGCGCT AGC^GCT 334 AGCGCT Eco81I 449 CCTCAGG CC^T[ACGT]AGG 697 CCTGAGG EcoNI 288 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 808 CCTACCCCAGG HindIII 935 AAGCTT A^AGCTT 1016 AAGCTT MaeII 825 ACGT A^CGT 914 ACGT NspI 479 GCATGC [GA]CATG^[CT] 1197 GCATGC SacI 298 GAGCTC GAGCT^C 1029 GAGCTC SfcI 969 CTACAG C^T[GA][CT]AG 1359 CTGTAG SphI 479 GCATGC GCATG^C 1197 GCATGC StuI 43 AGGCCT AGG^CCT 305 AGGCCT TaqI 83 TCGA T^CGA 359 TCGA TthHB8I 83 TCGA T^CGA 359 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI TfiI TseI Tsp45I TspRI XhoII XmnI