################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AlwI 669 GATCC GGATC(4/5) AocI 1117 CCTGAGG CC^T[ACGT]AGG BalI 1069 TGGCCA TGG^CCA BanII 4 GAGCCC G[GA]GC[CT]^C BbsI 727 GAAGAC GAAGAC(2/6) BpuAI 727 GAAGAC GAAGAC(2/6) BsaWI 125 ACCGGA [AT]^CCGG[AT] BsmBI 31 GAGACG CGTCTC(1/5) BsmI 1260 GAATGC GAATGC(1/-1) BspHI 1157 TCATGA T^CATGA BsrBI 655 GAGCGG CCGCTC(-3/-3) BsrFI 1304 GCCGGC [GA]^CCGG[CT] BstEII 1467 GGTGACC G^GT[ACGT]ACC BstPI 1467 GGTGACC G^GT[ACGT]ACC BstXI 1457 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 668 AGATCC [GA]^GATC[CT] Bsu36I 1117 CCTGAGG CC^T[ACGT]AGG Cfr10I 1304 GCCGGC [GA]^CCGG[CT] DrdI 289 GACATCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1117 CCTGAGG CC^T[ACGT]AGG EcoNI 159 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1467 GGTGACC G^GT[ACGT]ACC EcoT14I 662 CCATGG C^C[AT][AT]GG FseI 1303 GGCCGGCC GGCCGG^CC HindIII 1144 AAGCTT A^AGCTT MflI 668 AGATCC [GA]^GATC[CT] MluNI 1069 TGGCCA TGG^CCA MscI 1069 TGGCCA TGG^CCA MspA1I 541 CAGCTG C[AC]G^C[GT]G NaeI 1304 GCCGGC GCC^GGC NcoI 662 CCATGG C^CATGG NgoMIV 1304 GCCGGC G^CCGGC PflMI 1506 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 754 GGGACCC [GA]G^G[AT]CC[CT] PvuII 541 CAGCTG CAG^CTG RcaI 1157 TCATGA T^CATGA SapI 681 GCTCTTC GCTCTTC(1/4) ScaI 1319 AGTACT AGT^ACT StyI 662 CCATGG C^C[AT][AT]GG Van91I 1506 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 668 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BlpI 375 GCTCAGC GC^T[ACGT]AGC 1002 GCTGAGC Bpu1102I 375 GCTCAGC GC^T[ACGT]AGC 1002 GCTGAGC BsaI 794 GAGACC GGTCTC(1/5) 150 GGTCTC BssSI 1450 CACGAG CACGAG(-5/-1) 370 CTCGTG CelII 375 GCTCAGC GC^T[ACGT]AGC 1002 GCTGAGC CfoI 534 GCGC GCG^C 567 GCGC DpnII 669 GATC ^GATC 780 GATC DraII 754 GGGACCC [GA]G^G[ACGT]CC[CT] 1286 GGGGCCT DraIII 769 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 820 CACGCAGTG EaeI 816 CGGCCA [CT]^GGCC[GA] 1069 TGGCCA EcoO109I 754 GGGACCC [GA]G^G[ACGT]CC[CT] 1286 GGGGCCT HgaI 594 GACGC GACGC(5/10) 730 GACGC HhaI 534 GCGC GCG^C 567 GCGC HinP1I 534 GCGC G^CGC 567 GCGC MaeII 100 ACGT A^CGT 1540 ACGT MboI 669 GATC ^GATC 780 GATC NdeII 669 GATC ^GATC 780 GATC Sau3AI 669 GATC ^GATC 780 GATC SbfI 264 CCTGCAGG CCTGCA^GG 1545 CCTGCAGG SmlI 352 CTCAAG C^T[CT][GA]AG 850 CTCAAG Sse8387I 264 CCTGCAGG CCTGCA^GG 1545 CCTGCAGG XcmI 896 CCACGGAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1283 CCAGGGGCCTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DsaI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV PleI PstI RsaI Sau96I ScrFI SfaNI SfcI SmaI TfiI TseI Tsp45I TspRI XmaCI XmaI