Restriction map for
pF1KB8303
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbsI BcgI BciVI BfaI
BfrI BglI BlnI BpuAI BsaBI
Bsp1407I BspDI BsrBI BsrGI BsrI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
EarI EcoO65I EcoRI EcoRV EcoT22I
FspI HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I Tth111I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 1010 TCCGGA T^CCGGA
AcyI 1180 GACGCC G[GA]^CG[CT]C
Alw44I 1543 GTGCAC G^TGCAC
AocI 1411 CCTGAGG CC^T[ACGT]AGG
Aor51HI 641 AGCGCT AGC^GCT
ApaLI 1543 GTGCAC G^TGCAC
AvaI 1108 CTCGAG C^[CT]CG[GA]G
BbrPI 469 CACGTG CAC^GTG
BbvCI 1089 CCTCAGC CCTCAGC(-5/-2)
BglII 1004 AGATCT A^GATCT
BlpI 1513 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1513 GCTGAGC GC^T[ACGT]AGC
BsaHI 1180 GACGCC G[GA]^CG[CT]C
BsaWI 1010 TCCGGA [AT]^CCGG[AT]
BseAI 1010 TCCGGA T^CCGGA
BsiWI 614 CGTACG C^GTACG
BsmFI 996 GTCCC GGGAC(10/14)
BsoBI 1108 CTCGAG C^[CT]CG[GA]G
BspEI 1010 TCCGGA T^CCGGA
BspHI 1451 TCATGA T^CATGA
BssSI 676 CACGAG CACGAG(-5/-1)
Bsu36I 1411 CCTGAGG CC^T[ACGT]AGG
CelII 1513 GCTGAGC GC^T[ACGT]AGC
DraIII 1621 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1653 GACTGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 162 CGGCCG C^GGCCG
EclXI 162 CGGCCG C^GGCCG
Eco47III 641 AGCGCT AGC^GCT
Eco52I 162 CGGCCG C^GGCCG
Eco81I 1411 CCTGAGG CC^T[ACGT]AGG
FseI 30 GGCCGGCC GGCCGG^CC
HaeII 641 AGCGCT [GA]GCGC^[CT]
Hin1I 1180 GACGCC G[GA]^CG[CT]C
HincII 1297 GTCAAC GT[CT]^[GA]AC
HindII 1297 GTCAAC GT[CT]^[GA]AC
MroI 1010 TCCGGA T^CCGGA
MseI 497 TTAA T^TAA
PaeR7I 1108 CTCGAG C^TCGAG
PmaCI 469 CACGTG CAC^GTG
PmlI 469 CACGTG CAC^GTG
PpuMI 1694 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1000 CTGCAG CTGCA^G
RcaI 1451 TCATGA T^CATGA
Tru9I 497 TTAA T^TAA
XcmI 1706 CCAAGGTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1108 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 615 GTAC GT^AC
1155 GTAC
AflIII 893 ACATGT A^C[GA][CT]GT
1157 ACATGT
AlwI 867 GATCC GGATC(4/5)
1086 GATCC
BsaAI 469 CACGTG [CT]AC^GT[GA]
612 CACGTA
BseRI 1669 CTCCTC GAGGAG(10/8)
1741 GAGGAG
BsmBI 1324 GAGACG CGTCTC(1/5)
1573 GAGACG
BsmI 101 GAATGC GAATGC(1/-1)
977 GAATGC
BspLU11I 893 ACATGT A^CATGT
1157 ACATGT
BsrDI 69 CATTGC GCAATG(2/0)
1580 CATTGC
BsrFI 31 GCCGGC [GA]^CCGG[CT]
1311 GCCGGC
BstXI 302 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1250 CCAAGAAGATGG
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
1311 GCCGGC
DsaI 683 CCGTGG C^C[GA][CT]GG
803 CCACGG
EcoNI 1668 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1782 CCTCCCCCAGG
NaeI 31 GCCGGC GCC^GGC
1311 GCCGGC
NgoMIV 31 GCCGGC G^CCGGC
1311 GCCGGC
PflMI 36 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCACGTGCTGG
PvuII 1198 CAGCTG CAG^CTG
1522 CAGCTG
RsaI 615 GTAC GT^AC
1155 GTAC
SacI 739 GAGCTC GAGCT^C
1288 GAGCTC
SfcI 1000 CTGCAG C^T[GA][CT]AG
1590 CTACAG
SmlI 1108 CTCGAG C^T[CT][GA]AG
1600 CTCAAG
StuI 452 AGGCCT AGG^CCT
917 AGGCCT
TspRI 217 CACTG CA[GC]TG[ACGT][ACGT]^
1621 CACTG
Van91I 36 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCACGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AccII AciI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BclI BcnI BmyI BpmI BsaI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TthHB8I
XhoII