################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BcgI BciVI BfaI BfrI BglI BglII BlnI BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MluI MluNI MscI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 812 TCCGGA T^CCGGA Alw44I 940 GTGCAC G^TGCAC AlwNI 1507 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 940 GTGCAC G^TGCAC AvaI 565 CCCGAG C^[CT]CG[GA]G BamHI 735 GGATCC G^GATCC BbrPI 931 CACGTG CAC^GTG BbsI 1401 GAAGAC GAAGAC(2/6) BclI 1706 TGATCA T^GATCA BlpI 146 GCTCAGC GC^T[ACGT]AGC Bpu1102I 146 GCTCAGC GC^T[ACGT]AGC BpuAI 1401 GAAGAC GAAGAC(2/6) BsaAI 931 CACGTG [CT]AC^GT[GA] BseAI 812 TCCGGA T^CCGGA BseRI 1933 GAGGAG GAGGAG(10/8) BsmI 1116 GAATGC GAATGC(1/-1) BsoBI 565 CCCGAG C^[CT]CG[GA]G BspEI 812 TCCGGA T^CCGGA BspHI 29 TCATGA T^CATGA BsrDI 657 CATTGC GCAATG(2/0) BsrFI 275 GCCGGC [GA]^CCGG[CT] BstAPI 723 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 146 GCTCAGC GC^T[ACGT]AGC Cfr10I 275 GCCGGC [GA]^CCGG[CT] DraII 670 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 304 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 892 GACAAAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 670 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 782 CCTTGG C^C[AT][AT]GG FauI 1386 GCGGG CCCGC(4/6) FbaI 1706 TGATCA T^GATCA HgaI 990 GCGTC GACGC(5/10) MfeI 401 CAATTG C^AATTG MroI 812 TCCGGA T^CCGGA MseI 1562 TTAA T^TAA MunI 401 CAATTG C^AATTG NaeI 275 GCCGGC GCC^GGC NgoMIV 275 GCCGGC G^CCGGC PleI 1585 GACTC GAGTC(4/5) PmaCI 931 CACGTG CAC^GTG PmlI 931 CACGTG CAC^GTG PpuMI 670 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 103 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1633 CTGCAG CTGCA^G RcaI 29 TCATGA T^CATGA SacI 358 GAGCTC GAGCT^C ScaI 1370 AGTACT AGT^ACT SmlI 423 CTCAAG C^T[CT][GA]AG StyI 782 CCTTGG C^C[AT][AT]GG TaqI 239 TCGA T^CGA TfiI 230 GAATC G^A[AT]TC Tru9I 1562 TTAA T^TAA TthHB8I 239 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 62 GACGTC GACGT^C 223 GACGTC AccII 1393 CGCG CG^CG 1502 CGCG AcsI 211 AAATTT [GA]^AATT[CT] 756 AAATTC AflIII 932 ACGTGT A^C[GA][CT]GT 1739 ACGTGT ApoI 211 AAATTT [GA]^AATT[CT] 756 AAATTC BbeI 1091 GGCGCC GGCGC^C 1178 GGCGCC BbvCI 1849 CCTCAGC CCTCAGC(-5/-2) 1930 GCTGAGG BsaWI 68 ACCGGA [AT]^CCGG[AT] 812 TCCGGA BspMI 1529 ACCTGC ACCTGC(4/8) 1266 GCAGGT BsrBI 1029 CCGCTC CCGCTC(-3/-3) 643 GAGCGG BstUI 1393 CGCG CG^CG 1502 CGCG BstXI 600 CCAGAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1412 CCATCTTCATGG DsaI 1086 CCGTGG C^C[GA][CT]GG 1789 CCGTGG HinfI 230 GAATC G^A[ACGT]TC 1585 GACTC KasI 1091 GGCGCC G^GCGCC 1178 GGCGCC MvnI 1393 CGCG CG^CG 1502 CGCG NarI 1091 GGCGCC GG^CGCC 1178 GGCGCC PflMI 777 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1169 CCATCAACTGG PvuII 1852 CAGCTG CAG^CTG 1928 CAGCTG SfcI 954 CTACAG C^T[GA][CT]AG 1633 CTGCAG SfoI 1091 GGCGCC GGC^GCC 1178 GGCGCC SphI 728 GCATGC GCATG^C 1661 GCATGC Van91I 777 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1169 CCATCAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AocI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII EaeI Eco81I EcoNI EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI XhoII