##################################
# NO CUT ENZYMES (  222 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BclI        BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AciI            4  CCGC                 CCGC(-3/-1)
AluI           64  AGCT                 AG^CT
AspHI         116  GTGCTC               G[AT]GC[AT]^C
BamHI          12  GGATCC               G^GATCC
BciVI         123  GTATCC               GTATCC(6/5)
BglII         136  AGATCT               A^GATCT
BmyI          116  GTGCTC               G[AGT]GC[ACT]^C
BsaJI         128  CCAAGG               C^C[ACGT][ACGT]GG
BsiHKAI       116  GTGCTC               G[AT]GC[AT]^C
Bsp1286I      116  GTGCTC               G[AGT]GC[ACT]^C
BstF5I         93  CATCC                GGATG(2/0)
Cac8I          27  GCAAGC               GC[ACGT]^[ACGT]GC
DdeI          119  CTCAG                C^T[ACGT]AG
EarI          166  GAAGAG               CTCTTC(1/4)
EcoT14I       128  CCAAGG               C^C[AT][AT]GG
Fnu4HI          3  GCCGC                GC^[ACGT]GC
FokI           93  CATCC                GGATG(9/13)
HinfI          73  GAATC                G^A[ACGT]TC
ItaI            3  GCCGC                GC^[ACGT]GC
Ksp632I       166  GAAGAG               CTCTTC(1/4)
MnlI          169  GAGG                 CCTC(7/6)
NlaIII         69  CATG                 CATG^
NlaIV          12  GGATCC               GG[ACGT]^[ACGT]CC
SfaNI         196  GCATC                GCATC(5/9)
StyI          128  CCAAGG               C^C[AT][AT]GG
TfiI           73  GAATC                G^A[AT]TC
XcmI           59  CCAACAGCTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwI           13  GATCC                GGATC(4/5)
               12  GGATC               
BstYI          12  GGATCC               [GA]^GATC[CT]
              136  AGATCT              
DpnII          13  GATC                 ^GATC
              137  GATC                
MboI           13  GATC                 ^GATC
              137  GATC                
MflI           12  GGATCC               [GA]^GATC[CT]
              136  AGATCT              
NdeII          13  GATC                 ^GATC
              137  GATC                
Sau3AI         13  GATC                 ^GATC
              137  GATC                
XhoII          12  GGATCC               [GA]^GATC[CT]
              136  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MboII