Restriction map for
pF1KB8313
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tru9I Tth111I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 853 GTCTAC GT^[AC][GT]AC
AccIII 440 TCCGGA T^CCGGA
BglI 56 GCCGTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1037 AGATCT A^GATCT
BpmI 462 CTCCAG CTGGAG(16/14)
BsaI 1009 GAGACC GGTCTC(1/5)
BsaWI 440 TCCGGA [AT]^CCGG[AT]
BseAI 440 TCCGGA T^CCGGA
BsgI 204 GTGCAG GTGCAG(16/14)
BsiEI 54 CGGCCG CG[GA][CT]^CG
BsmAI 1009 GAGAC GTCTC(1/5)
BsmFI 298 GTCCC GGGAC(10/14)
BspEI 440 TCCGGA T^CCGGA
BspHI 1064 TCATGA T^CATGA
BspMI 548 ACCTGC ACCTGC(4/8)
BssSI 340 CACGAG CACGAG(-5/-1)
EaeI 54 CGGCCG [CT]^GGCC[GA]
EagI 54 CGGCCG C^GGCCG
EarI 532 CTCTTC CTCTTC(1/4)
EclXI 54 CGGCCG C^GGCCG
Eco52I 54 CGGCCG C^GGCCG
HaeII 884 AGCGCC [GA]GCGC^[CT]
HphI 309 GGTGA GGTGA(8/7)
Ksp632I 532 CTCTTC CTCTTC(1/4)
MaeIII 811 GTCAC ^GT[ACGT]AC
MroI 440 TCCGGA T^CCGGA
MspA1I 691 CAGCTG C[AC]G^C[GT]G
NspI 593 ACATGC [GA]CATG^[CT]
PleI 1068 GAGTC GAGTC(4/5)
PvuII 691 CAGCTG CAG^CTG
RcaI 1064 TCATGA T^CATGA
SapI 531 GCTCTTC GCTCTTC(1/4)
ScaI 734 AGTACT AGT^ACT
SmaI 251 CCCGGG CCC^GGG
StuI 515 AGGCCT AGG^CCT
Tsp45I 811 GTCAC ^GT[GC]AC
Tsp509I 189 AATT ^AATT
XcmI 104 CCAGGGCGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 251 CCCGGG C^CCGGG
XmaI 251 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 231 GATCC GGATC(4/5)
315 GATCC
BanII 373 GAGCTC G[GA]GC[CT]^C
529 GAGCTC
BcnI 251 CCCGG CC^[GC]GG
252 CCGGG
HapII 252 CCGG C^CGG
441 CCGG
HgaI 43 GACGC GACGC(5/10)
804 GCGTC
HpaII 252 CCGG C^CGG
441 CCGG
MspI 252 CCGG C^CGG
441 CCGG
NciI 251 CCCGG CC^[GC]GG
252 CCGGG
PaeR7I 157 CTCGAG C^TCGAG
471 CTCGAG
PpuMI 137 AGGACCT [GA]G^G[AT]CC[CT]
296 GGGTCCC
SacI 373 GAGCTC GAGCT^C
529 GAGCTC
SfaNI 348 GATGC GCATC(5/9)
555 GATGC
TfiI 564 GAATC G^A[AT]TC
939 GAATC
XhoI 157 CTCGAG C^TCGAG
471 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BbvI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
ItaI MaeI MboI MboII MflI
MnlI MvaI MvnI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI TaqI
TseI TspRI TthHB8I XhoII