Restriction map for
pF1KB8314
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI KspI
MaeII MamI MfeI MluI MseI
MunI MvnI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 545 TCCGGA T^CCGGA
AfaI 765 GTAC GT^AC
AlwNI 43 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 393 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 157 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 638 TGCGCA TGC^GCA
AvrII 777 CCTAGG C^CTAGG
BbeI 14 GGCGCC GGCGC^C
BlnI 777 CCTAGG C^CTAGG
BsaWI 545 TCCGGA [AT]^CCGG[AT]
BseAI 545 TCCGGA T^CCGGA
BseRI 901 CTCCTC GAGGAG(10/8)
Bsp1407I 764 TGTACA T^GTACA
BspEI 545 TCCGGA T^CCGGA
BspHI 101 TCATGA T^CATGA
BsrFI 1044 GCCGGC [GA]^CCGG[CT]
BsrGI 764 TGTACA T^GTACA
BssSI 19 CTCGTG CACGAG(-5/-1)
Cfr10I 1044 GCCGGC [GA]^CCGG[CT]
DraIII 445 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 965 CCATGG C^C[GA][CT]GG
EarI 1009 CTCTTC CTCTTC(1/4)
EcoNI 36 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 638 TGCGCA TGC^GCA
HincII 949 GTCAAC GT[CT]^[GA]AC
HindII 949 GTCAAC GT[CT]^[GA]AC
HindIII 119 AAGCTT A^AGCTT
HinfI 157 GACTC G^A[ACGT]TC
KasI 14 GGCGCC G^GCGCC
Ksp632I 1009 CTCTTC CTCTTC(1/4)
MboII 1010 TCTTC GAAGA(8/7)
MroI 545 TCCGGA T^CCGGA
NaeI 1044 GCCGGC GCC^GGC
NarI 14 GGCGCC GG^CGCC
NcoI 965 CCATGG C^CATGG
NgoMIV 1044 GCCGGC G^CCGGC
PflFI 157 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 444 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 157 GACTC GAGTC(4/5)
PpuMI 740 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 101 TCATGA T^CATGA
RsaI 765 GTAC GT^AC
SexAI 446 ACCTGGT A^CC[AT]GGT
SfoI 14 GGCGCC GGC^GCC
SmlI 895 CTCAAG C^T[CT][GA]AG
SspBI 764 TGTACA T^GTACA
StuI 395 AGGCCT AGG^CCT
Tth111I 157 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 444 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 393 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 14 GGCGCC G[GA]^CG[CT]C
142 GGCGTC
AlwI 384 GATCC GGATC(4/5)
456 GATCC
AocI 137 CCTCAGG CC^T[ACGT]AGG
878 CCTCAGG
BalI 866 TGGCCA TGG^CCA
1076 TGGCCA
BcnI 153 CCCGG CC^[GC]GG
837 CCGGG
BfaI 706 CTAG C^TAG
778 CTAG
BglI 6 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
876 GCCCTCAGGGC
BglII 260 AGATCT A^GATCT
374 AGATCT
BsaHI 14 GGCGCC G[GA]^CG[CT]C
142 GGCGTC
BsaI 685 GAGACC GGTCTC(1/5)
334 GGTCTC
BsgI 919 CTGCAC GTGCAG(16/14)
1039 GTGCAG
BsmFI 74 GGGAC GGGAC(10/14)
962 GTCCC
Bsu36I 137 CCTCAGG CC^T[ACGT]AGG
878 CCTCAGG
DraII 44 AGGCCCT [GA]G^G[ACGT]CC[CT]
740 AGGACCT
Eco81I 137 CCTCAGG CC^T[ACGT]AGG
878 CCTCAGG
EcoO109I 44 AGGCCCT [GA]G^G[ACGT]CC[CT]
740 AGGACCT
HgaI 143 GCGTC GACGC(5/10)
1061 GCGTC
Hin1I 14 GGCGCC G[GA]^CG[CT]C
142 GGCGTC
MaeI 706 CTAG C^TAG
778 CTAG
MaeIII 163 GTCAC ^GT[ACGT]AC
1063 GTCAC
MluNI 866 TGGCCA TGG^CCA
1076 TGGCCA
MscI 866 TGGCCA TGG^CCA
1076 TGGCCA
MslI 561 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
936 CACCAAAGTG
MspA1I 537 CAGCGG C[AC]G^C[GT]G
1056 CCGCTG
NciI 153 CCCGG CC^[GC]GG
837 CCGGG
PshAI 232 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
955 GACTTCTGTC
PstI 289 CTGCAG CTGCA^G
418 CTGCAG
Tsp45I 163 GTCAC ^GT[GC]AC
1063 GTCAC
XcmI 437 CCAAGAACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
473 CCAGCTCCCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BanII BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI TspRI
TthHB8I XhoII